Cell culture from fish is an excellent tool for many advanced applications. The cell lines derived from a particular fish species are more appropriate for studying the viruses reported from that species and studying virus-host interactions (Crane and Hyatt, 2011 and Pandey, 2013). It is an excellent alternative to obtain good quality chromosome preparations in cytogenetic studies of small and large species. With the establishment of a cell bank available at any time and in case of a repetition of cytogenetic methodologies, it is not necessary to go back to the field for new individuals (Paim et al., 2018). Fish cell lines are appropriate for in vitro assays, since they are believed to retain fish-specific traits in their metabolism of chemicals (Schenel et al., 2015). Cell cultures lack in vivo complexities such as bioaccumulation and depuration. Finally, experiments with cell ARTICLE INFO