Establishment of a cell culture system and characterization of the primary cultures from different organs of the Nile tilapia Oreochromis niloticus

Abstract

Cell culture from fish is an excellent tool for many advanced applications. The cell lines derived from a particular fish species are more appropriate for studying the viruses reported from that species and studying virus-host interactions (Crane and Hyatt, 2011 and Pandey, 2013). It is an excellent alternative to obtain good quality chromosome preparations in cytogenetic studies of small and large species. With the establishment of a cell bank available at any time and in case of a repetition of cytogenetic methodologies, it is not necessary to go back to the field for new individuals (Paim et al., 2018). Fish cell lines are appropriate for in vitro assays, since they are believed to retain fish-specific traits in their metabolism of chemicals (Schenel et al., 2015). Cell cultures lack in vivo complexities such as bioaccumulation and depuration. Finally, experiments with cell ARTICLE INFO

Feminization and Molecular Sexing of the Nile Tilapia (Oreochromis niloticus, L) Through Sex Reversal Hormone Diethylstilbestrol and Molecular Markers

“…Fish and its products represent an important source of proteins and essential micronutrients for balanced nutrition (Srinivasan et al, 2015, Hashem et al, 2020Abdel-Hamid et al, 2023). Among cultivated fish, tilapia nilotica (Oreochromis niloticus) has many advantages, for example, the tolerance to adverse conditions of the environment; it can live at low oxygen conditions and somewhat gain fast growth.…”