Aim: This study assessed the biocorrosive capacity of two bacteria: Desulfovibrio desulfuricans and Desulfovibrio fairfieldensis on endodontic files, as a preliminary step in the development of a biopharmaceutical, to facilitate the removal of endodontic file fragments from root canals. Materials and methods:In the first stage, the corrosive potential of the artificial saliva medium (ASM), modified Postgate E medium (MPEM), 2.5 % sodium hypochlorite (NaOCl) solution and white medium (WM), without the inoculation of bacteria was assessed by immersion assays. In the second stage, test samples were inoculated with the two species of sulphur-reducing bacteria (SRB) on ASM and modified artificial saliva medium (MASM). In the third stage, test samples were inoculated with the same species on MPEM, ASM and MASM. All test samples were viewed under an infinite focus Alicona microscope. Clinical significance: This study describes the initial development of a biopharmaceutical to facilitate the removal of endodontic file fragments from root canals, which can be successfully implicated in endodontic therapy in order to avoiding parendodontic surgery or even tooth loss in such events. Results

Uma auxiliar de laboratório infectou-se acidentalmente, com cercárias de Schistosoma mansoni, cepa LE, mantida rotineiramente em nossos laboratórios. Decorridos 5 meses, o exame parasitológico de fezes revelou 108 ovos/g . A pacientefoi tratada com oxamniquine, porém a infecção continuou ativa (6 ovos/g). Foi então obtido o isolado SSF mantido no modelo Biomphalaria glabrata - camundongo albino. Os resultados obtidos no estudo comparativo, entre o isolado SSF e a cepa LE, que lhe deu origem, mostraram que a duração do período pré-patente e o índice de infectividade em camundongos, bem como a resposta aos agentes esquistossomicidas (hycanthone, oxamniquine epraziquantel) não apresentaram diferenças estatisticamente significativas. Por outro lado, o número de miracídios obtidos dos intestinos e fígados dos camundongos infectados foi o dobro com a cepa LE, quando comparados com aquele do isolado SSF. Também a variação do peso dos animais foi bastante diferente. Concluiu-se que apenas uma passagem pelo hospedeiro humano não mudou substancialmente as características da cepa estudada.

Aim: The aim of this study is to correlate the A1C values with the OGTT in individuals without type 2 Diabetes (DM) but with impaired fasting glucose (IFG) (FPG: 5.6-6.9mmol/l). Methods:We investigated 119 subjects with IFG divided according to A1C levels (<5.7%; 5.7-6.4%, ≥6.5%) and analyzed the correlation among A1C, FPG and 2-hPG by Pearson's coefficient. Kappa coefficient was used to test agreement between A1C and 2-hPG for the diagnosis of DM. Results:The average age of the subjects was 54.2 ± 14.6 years, the average BMI was 30.2 ±5.5 and 70.5% were women. While levels of A1C ≥6.5% were associated to alterations in the 2-hPG in 86.7%, only 28.6% of these patients had 2-hPG >11.1mmol/l. Furthermore, 31.6% of the patients with DM diagnosed by the OGTT had A1C levels ≥6.5% and 64.3% of the patients with A1C < 5.7% had a normal OGTT. The Kappa coefficient of reliability between A1C and 2-hPG to diagnose DM was 0.71. Conclusion:A1C is a useful tool in evaluating IFG patients and correlates better to 2-hPG than to FPG. Yet, the agreement between A1C and OGTT to diagnose DM in this group was moderate (71%). It may be too soon to consider A1C as a substitute exam for OGTT.