Biocorrosion of Endodontic Files through the Action of Two Species of Sulfate-reducing Bacteria: Desulfovibrio desulfuricans and Desulfovibrio fairfieldensis

Abstract

Aim: This study assessed the biocorrosive capacity of two bacteria: Desulfovibrio desulfuricans and Desulfovibrio fairfieldensis on endodontic files, as a preliminary step in the development of a biopharmaceutical, to facilitate the removal of endodontic file fragments from root canals. Materials and methods:In the first stage, the corrosive potential of the artificial saliva medium (ASM), modified Postgate E medium (MPEM), 2.5 % sodium hypochlorite (NaOCl) solution and white medium (WM), without the inoculation of bacteria was assessed by immersion assays. In the second stage, test samples were inoculated with the two species of sulphur-reducing bacteria (SRB) on ASM and modified artificial saliva medium (MASM). In the third stage, test samples were inoculated with the same species on MPEM, ASM and MASM. All test samples were viewed under an infinite focus Alicona microscope. Clinical significance: This study describes the initial development of a biopharmaceutical to facilitate the removal of endodontic file fragments from root canals, which can be successfully implicated in endodontic therapy in order to avoiding parendodontic surgery or even tooth loss in such events. Results

Initial cytotoxicity assays of media for sulfate-reducing bacteria: An endodontic biopharmaceutical product under development

“…Therefore, initial cytotoxicity tests can present limitations when correlated with the clinical situations, however they are valid to determine the biological behavior of the materials and/or their components 17,23,25) . The finding of the current study in conjunction with previous results that demonstrated biocorrosion of SRB on endodontic files 28) , besides the fact that this group of bacteria can be found in oral microbiota of healthy patients 29,30) contribute to the probable clinical aplication of this biopharmaceutical product (BACCOR) for dental use.…”

Sulfate-Reducing Bacteria: Biofilm Formation and Corrosive Activity in Endodontic Files

“…This biopharmaceutical is still under development to aid in the removal of fractured endodontic files from root canals. Previous studies have demonstrated that D. desulfuricans and D. fairfieldensis are capable of promoting biocorrosion of endodontic files [ 10 ], and cytotoxicity tests have shown that the inoculation vehicle (used in this work) is biocompatible [ 11 ].…”

Assessing the Potential Association Between Microbes and Corrosion of Intra-Oral Metallic Alloy-Based Dental Appliances Through a Systematic Review of the Literature

“…Of the 25 articles selected 5 were from the USA (Vaidyanathan et al, 1991;Chang et al, 2003;Oshida et al, 2003;Pozhitkov et al, 2015;Sridhar et al, 2016), 3 each were from Japan (Fukushima et al, 2014;Kameda et al, 2014Kameda et al, , 2019, Portugal (Souza et al, 2010;Proença et al, 2015;Figueiredo-Pina et al, 2019), France (Laurent et al, 2001;Mabilleau et al, 2006;Jorand et al, 2015), Poland (Mystkowska, 2016;Cwalina et al, 2017;Mystkowska et al, 2017), 2 each from Brazil (Heggendorn et al, 2015;Zavanelli et al, 2015) and China (Zhang et al, 2013;Lu et al, 2017), 1 each from Croatia (Pavlic et al, 2019), Spain (Díaz et al, 2018), Italy (Lucchetti et al, 2015), and India (Maruthamuthu et al, 2005).…”

“…Vital data including the assessed metallic alloy, the microorganisms, the methodology employed for detecting the microbe, and for assessing the corrosion, the statistical data, and the inferences drawn were extracted from all the included studies (Table 1). Titanium (Ti) was assessed in 16 studies (Oshida et al, 2003;Maruthamuthu et al, 2005;Mabilleau et al, 2006;Souza et al, 2010;Fukushima et al, 2014;Kameda et al, 2014;Jorand et al, 2015;Pozhitkov et al, 2015;Mystkowska, 2016;Sridhar et al, 2016;Díaz et al, 2018;Figueiredo-Pina et al, 2019), stainless steel (SUS) in five studies (Chang et al, 2003;Oshida et al, 2003;Maruthamuthu et al, 2005;Kameda et al, 2014;Heggendorn et al, 2015;Mystkowska et al, 2017), nickel (NiCr) and cobalt-chromium (CoCr) alloys in seven studies (Laurent et al, 2001;Chang et al, 2003;Oshida et al, 2003;Lucchetti et al, 2015;Proença et al, 2015;Mystkowska, 2016;Lu et al, 2017), neodymium-iron, zirconia (Figueiredo-Pina et al, 2019, amalgam and copper aluminum alloy (Zavanelli et al, 2015) and precious metal alloys (Vaidyanathan et al, 1991) were each assessed on one study. The bacteria that were studied include probiotic bacteria Lactobacillus reuteri (Pavlic et al, 2019), Streptococcus (S.) mutans (Chang et al, 2003;Oshida et al, 2003;Souza et al, 2010;Fukushima et al, 2014;…”

“…S.mitis has also been shown to be corrosive toward Ti alloy (Mabilleau et al, 2006). Sulfate-reducing bacteria (Heggendorn et al, 2015;Jorand et al, 2015;Mystkowska, 2016;Cwalina et al, 2017;Mystkowska et al, 2017) have been shown to corrode SUS and Ti alloys. Actinomyces (Vaidyanathan et al, 1991;Laurent et al, 2001;Zhang et al, 2013) caused corrosion of NiCr and other precious metal alloys.…”