Initial cytotoxicity assays of media for sulfate-reducing bacteria: An endodontic biopharmaceutical product under development

Heggendorn, Fabiano Luiz; Silva, Gabriela Cristina de Carvalho; Cardoso, Elisama Azevedo; Castro, Helena C.; Gonçalves, Lúcio Souza; Dias, Eliane Pedra; Lione, Viviane de Oliveira Freitas; Lutterbach, Márcia Teresa Soares

doi:10.4012/dmj.2015-360

Cited by 4 publications

(3 citation statements)

References 17 publications

(23 reference statements)

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“…This biopharmaceutical is still under development to aid in the removal of fractured endodontic files from root canals. Previous studies have demonstrated that D. desulfuricans and D. fairfieldensis are capable of promoting biocorrosion of endodontic files [ 10 ], and cytotoxicity tests have shown that the inoculation vehicle (used in this work) is biocompatible [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Sulfate-Reducing Bacteria: Biofilm Formation and Corrosive Activity in Endodontic Files

Heggendorn

Fraga

Ferreira

et al. 2018

International Journal of Dentistry

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Aim This study describes the biofilm formation and the corrosive capacity of sulfate-reducing bacteria (SRB) on the metallic structure of used endodontic files. Methods Sulfate-reducing bacteria (SRB) (Desulfovibrio desulfuricans oral and Desulfovibrio fairfieldensis or D. desulfuricans environmental) were inoculated into the culture media (Postgate C culture medium or modified Postgate E culture medium). The biocorrosive potential of these bacteria will be an important component of a biopharmaceutical under development called BACCOR. Afterwards, four used endodontic files (UEFs) were separately inoculated into a specific culture media for 445 days at 30°C in an incubator. The four UEFs were placed in a scanning electron microscope (SEM) and analyzed by the energy-dispersive X-ray spectrometry (EDS). Results The confocal laser scanning microscopic images indicate the presence of biofilm in the four samples. The SEM and SEM-EDS revealed the presence of rough, irregular structures adhering along the metallic surface of the used endodontic files, suggesting a mature calcified biofilm with a high concentration of Ca, P, C, and S. Conclusion The formation of SRB biofilms on used endodontic files shows characteristics that may contribute to the biocorrosion of these files, and the results may also provide complementary data for a biopharmaceutical, which is still under development to assist in the removal of fractured endodontic files inside root channels.

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Section: Introductionmentioning

confidence: 99%

Sulfate-Reducing Bacteria: Biofilm Formation and Corrosive Activity in Endodontic Files

Heggendorn

Fraga

Ferreira

et al. 2018

International Journal of Dentistry

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“…A 60-day immersion period was chosen for the study based on the reports of Isa et al [ 17 ] that the SRB in anaerobic reactors were most active between 11 and 24 days after which the activity decreased with a reduction in sulfide production after 50 days as a supersaturation with FeS2 will inhibit the further corrosion of steel [ 18 ]. The corrosive property of SRB has been experimentally elicited by other authors like Heggendorn et al [ 4 , 19 ] who showed the biofilm interaction with the metallic surface of endodontic files and the consequent corrosion. The results of our study are in line with their study wherein we identified localized corrosion induced by SRB of genus Desulfovibrio in stainless steel brackets and wires, NiTi, TMA wires, and Titanium mini-implants.…”

Section: Discussionmentioning

confidence: 97%

In-Vitro Assessment of the Corrosion Potential of an Oral Strain of Sulfate-Reducing Bacteria on Metallic Orthodontic Materials

Gopalakrishnan¹,

Thiagarajan

Felicita

et al. 2022

IJERPH

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Aim: Orthodontic literature is scant when it comes to microbial corrosion. The oral prevalence of many bacteria which are capable of causing microbial corrosion is reported in the dental literature. The aim of this study is to experimentally determine the corrosive potential of an oral strain of Sulfate-reducing bacteria. Materials and Methods: Stainless steel (SS) bracket, stainless steel archwire, NiTi archwire, Titanium molybdenum (TMA) archwire, and titanium miniscrew were immersed in five media which included Artificial saliva (group I), Sulfate rich artificial saliva (group II), API agar medium specific for SRB (group III), AS + API medium+ bacterial strain (group IV), SRAS+ API medium+ bacterial strain (group V). The materials were then subjected to Scanning electron microscopy and energy-dispersive X-ray analysis (EDX). Results: Materials in groups I, II, and III did not show any surface changes whereas materials in groups IV and V which contained the bacteria showed surface changes which were erosive patches suggestive of corrosion. EDX analyses were in line with similar findings. Conclusion: This in vitro study suggested that the oral strain of Sulfate-reducing bacteria was able to induce corrosive changes in the experimental setup.

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“…TDN is a type of nitrate‐reducing, sulfide‐oxidizing bacteria that can induce the biological oxidation of sulfide while reducing the nitrate to nitrogen in anaerobic condition (Qiu et al , 2015). During growth, TDN produces nitrites, oxynitride, NO 2 , NO, N 2 O and nitrogen while also secreting extracellular enzymes and polysaccharides (Heggendorn et al , 2016). When TDN is apoptosis, the enzyme is released due to cell rupture.…”

Section: Introductionmentioning

confidence: 99%

Study on enzyme activity inhibition mechanism of Thiobacillus denitrification intracellular enzyme to sulfate-reducing bacteria intracellular enzyme

Qiu

Tian

Zhang

et al. 2022

ACMM

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Purpose Sulfate-reducing bacteria (SRB) are recognized by scholars as the most important class of bacteria leading to corrosion of metal materials. It is important to use the properties of microorganisms to inhibit the growth of SRB in the corrosion protection of metal materials and to protect the environment. Design/methodology/approach In this work, the behavior of anaerobic Thiobacillus denitrificans (TDN) intracellular enzyme inhibition of SRB corrosion of EH36 steel was investigated with electrochemical impedance spectroscopy, biological detection technology and X-ray photoelectron spectroscopy. Findings Results showed that the SRB crude intracellular enzyme affected the corrosion behavior of EH36 steel greatly and the purified TDN intracellular enzyme inhibits SRB intracellular enzyme corrosion to EH36 steel. Originality/value A perfect enzyme activity inhibition mechanism will provide theoretical guidance for the selection and application of anticorrosion microorganisms, which is of scientific significance in the field of microbial anticorrosion research.

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Initial cytotoxicity assays of media for sulfate-reducing bacteria: An endodontic biopharmaceutical product under development

Cited by 4 publications

References 17 publications

Sulfate-Reducing Bacteria: Biofilm Formation and Corrosive Activity in Endodontic Files

Sulfate-Reducing Bacteria: Biofilm Formation and Corrosive Activity in Endodontic Files

In-Vitro Assessment of the Corrosion Potential of an Oral Strain of Sulfate-Reducing Bacteria on Metallic Orthodontic Materials

Study on enzyme activity inhibition mechanism of Thiobacillus denitrification intracellular enzyme to sulfate-reducing bacteria intracellular enzyme

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