Natural selection as a mechanism of evolution is a central concept in biology; yet, most nonbiology-majors do not thoroughly understand the theory even after instruction. Many alternative conceptions on this topic have been identified, indicating that the job of the instructor is a difficult one. This article presents a new diagnostic test to assess students' understanding of natural selection. The test items are based on actual scientific studies of natural selection, whereas previous tests have employed hypothetical situations that were often misleading or oversimplified. The Conceptual Inventory of Natural Selection (CINS) is a 20-item multiple choice test that employs common alternative conceptions as distractors. An original 12-item version of the test was field-tested with 170 nonmajors in 6 classes and 43 biology majors in 1 class at 3 community colleges. The test scores of one subset of nonmajors (n ¼ 7) were compared with the students' performances in semistructured interviews. There was a positive correlation between the test scores and the interview scores. The current 20-item version of the CINS was field-tested with 206 students in a nonmajors' general biology course. The face validity, internal validity, reliability, and readability of the CINS are discussed. Results indicate that the CINS will be a valuable tool for instructors. ß 2002 Wiley Periodicals, Inc. J Res Sci Teach 39: 952-978, 2002 Natural selection is the principal mechanism of evolution, and the theory of evolution is of great importance as a unifying theory in biology education according to the National Science Standards (National Research Council, 1996). Yet, natural selection is misunderstood by many students. The litany of alternative conceptions regarding natural selection and evolution is long (Mayr,

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Embryonic mortality and abnormal placental morphology have been reported by most researchers studying nuclear transfer (NT), and it now is accepted that placental anomalies and poor development of cloned embryos are related. As early as day 50 of gestation, cloned bovine concepti exhibit poor structural organization of the developing placentomes. These experiments were designed to identify alterations in maternal-fetal interactions during establishment of the placentas of NT-derived embryos at day 30 of gestation. Bovine NT embryos were produced using cultured fibroblast cells from a single Hereford donor cow, and control embryos were derived from in vitro fertilization (IVF). Following in vivo culture in ligated sheep oviducts, day-8 blastocysts were transferred to synchronized recipient heifers. Tissues recovered from viable day-30 pregnancies were analyzed by real-time RT-PCR, immunohistochemistry, and quantitative histological techniques. Immunoperoxidase staining of caruncular tissue from NT- and IVF-derived pregnancies revealed no significant differences in expression of the extracellular matrix proteins, collagen type IV and laminin, or the receptor subunits, integrins alpha1 and alpha3, suggesting that altered expression of these proteins at day 30 of gestation is not a primary cause of abnormal placentome structure in cloned concepti. Percentage of binucleate cells (BNC) within the trophoblast also was similar in NT- and IVF-derived pregnancies; however, expression of the BNC-specific placental lactogen (PL) transcript was elevated in NT-derived concepti (p < 0.05). These results indicate that regulation of PL transcription was altered in cloned day-30 placental tissues, suggesting the presence of irregular fetal-maternal signaling patterns that might undermine continued development of NT-derived concepti.

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Embryos were surgically flushed from goats and sheep on d 6 and 7, respectively, following the first day of estrus (d 0). After enzymatic removal of the zonae pellucidae, inner cell masses were isolated from caprine blastocysts by immunosurgery. The intact inner cell masses were injected into ovine blastocysts with the aid of a micromanipulator. Twenty-two manipulated blastocysts were surgically transferred into 12 ovine recipients. Nine ewes gave birth to a total of 13 young (59% embryo survival). Ten were classified by serum electrophoretic assays or karyotypes as lambs, one as a kid, and two as interspecific chimeras.