2-hydroxyglutarate (2HG) is produced in gliomas with mutations of isocitrate dehydrogenases (IDH) 1 and 2. The 1H resonances of the J-coupled spins of 2HG are extensively overlapped with signals from other metabolites. Here we report a comparative study at 3T of the utility of the PRESS (point-resolved spectroscopy) sequence with a standard short TE (35 ms) and a long TE (97 ms) which had been theoretically designed for detecting the 2HG 2.25 ppm resonance. The performance of the methods is evaluated using data from phantoms, 7 healthy volunteers, and 22 subjects with IDH-mutated gliomas. The results indicate that TE = 97 ms provides higher detectability of 2HG than TE = 35 ms, and that this improved capability is gained when data are analyzed with basis spectra that include the effects of the volume localizing radio-frequency and gradient pulses.
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