Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS), and fibromyalgia (FM) are two chronic complex diseases with overlapping symptoms affecting multiple systems and organs over time. Due to the absence of validated biomarkers and similarity in symptoms, both disorders are misdiagnosed, and the comorbidity of the two is often unrecognized. Our study aimed to investigate the expression profiles of 11 circulating miRNAs previously associated with ME/CFS pathogenesis in FM patients and individuals with a comorbid diagnosis of FM associated with ME/CFS (ME/CFS + FM), and matched sedentary healthy controls. Whether these 11 circulating miRNAs expression can differentiate between the two disorders was also examined. Our results highlight differential circulating miRNAs expression signatures between ME/CFS, FM and ME/CFS + FM, which also correlate to symptom severity between ME/CFS and ME/CFS + FM groups. We provided a prediction model, by using a machine-learning approach based on 11 circulating miRNAs levels, which can be used to discriminate between patients suffering from ME/CFS, FM and ME/CFS + FM. These 11 miRNAs are proposed as potential biomarkers for discriminating ME/CFS from FM. The results of this study demonstrate that ME/CFS and FM are two distinct illnesses, and we highlight the comorbidity between the two conditions. Proper diagnosis of patients suffering from ME/CFS, FM or ME/CFS + FM is crucial to elucidate the pathophysiology of both diseases, determine preventive measures, and establish more effective treatments.

This paper presents the results of aphid species monitoring in the seed potato crop during the growing season using Moerike aphids trap in 2020 and 2021 at INCDCSZ Brașov. Aphids control as direct pests is rarely justified in potato crop but absolutely necessary to prevent indirect damages caused by infecting potato plants with viruses transmitted by aphids. The aphids monitoring in seed potato crops is absolutely necessary to reduce the risk of the appearance and development of aphid populations, implicitly to decrease the incidence of viral contamination. During the two years of study, winged aphids were collected using yellow water traps¸ The vector aphid species were identified from the total of collected aphids. The identification resulted in a number of 20 vector species (682 - total number of individuals) in 2020 and 24 vector species (1843 - total number of individuals) in 2021.

Thirteen potato genotypes with different level of resistance to potato late blight (Phytophthora infestans) pathogen were characterized using non-invasive method to determinate the chlorophyll content and the late blight influence. Field experiment was conducted to NIRDPS Brasov, Romania between 2021-2022. During the vegetation were applied the usual maintenance works (hilling, herbicides), including three treatments for Colorado beetle unless late blight control with fungicides. With SPAD 502 Plus meter (Chlorophyll Meter) were carried out 3 determinations on the leaves of the middle level of 3 plants from each genotype taken into the study. For the evaluation of the results, analysis of variance and Pearson’s correlation analysis were used. In 2021, following the measurements, closely negative correlations were observed between SPAD values and genotypes (-0.058), respectively the date of the observations (-0.126), while in 2022, the correlation was negative between the values regarding the date of the observations (-0.065), but positive at genotypes level (0.030).