DDT is a hydrophobic organic pollutant, which can be bio-accumulated in nature and have adverse consequences on the physical condition of humans and animals. This study investigated the relationship between the white-rot fungus Pleurotus eryngii and biosurfactantproducing bacterium Ralstonia pickettii associated with the degradation of DDT. The effects of R. pickettii on fungal development were examined using in vitro confrontation assay on a potato dextrose agar (PDA) medium. R. pickettii culture was added to the P. eryngii culture at 1, 3, 5, 7, and 10 ml (1 ml ≈ 1.44 × 10 1 3 CFU). After 7 d incubation, about 43% of the initial DDT (12.5 µM) was degraded by the P. eryngii culture only. The augmentation of 7 ml of R. pickettii culture revealed a more highly optimized synergism with DDT degradation being approximately 78% and the ratio of optimization 1.06. According to the confrontational assay, R. pickettii promoted the growth of P. eryngii towards the bacterial colony, with no direct contact between the bacterial cells and mycelium (0.71 cm/day). DDD (1,1-dichloro-2,2-bis(4chlorophenyl) ethane), DDE (1,1-dichloro-2,2-bis(4-chlorophenyl) ethylene), and DDMU (1chloro-2,2-bis(4-chlorophenyl) ethylene) were identified as metabolic products, indicating that the R. pickettii could enhance the DDT biodegradation by P. eryngii.

Kerang Bakau (<em>Geloina coaxans</em>) merupakan salah satu jenis gastropoda yang diduga memiliki komponen bioaktif yang dapat digunakan sebagai antibakteri. Tujuan penelitian ini yaitu mengeksplorasi potensi kerang bakau (<em>G. coaxans</em>) sebagai sumber bahan alami antibakteri terhadap Vibrio parahaemolyticus. Aktivitas antibakteri ekstrak <em>G. coaxans</em> di uji dengan metode difusi pada konsentrasi 10, 50 dan 100 mg/mL, serta identifikasi senyawa aktif. Hasil penelitian menunjukan rendemen ekstrak etanol <em>G. coaxans</em> yaitu 8,50%, senyawa fitokomia ekstrak etanol <em>G. coaxans</em> terdiri dar alkaloid, tanin, flavonoids, saponin, triterpenoid dan steroid. Aktivitas antibakteri menunjukkan ekstrak etanol <em>G. coaxans</em> memiliki zona hambat sebesar 25,05 pada konsentrasi 100 mg/mL terhadap <em>V. parahaemolyticus</em>.

Ikan cupang salah satu ikan hias primadona karena memiliki nilai ekonomis yang tinggi. Ikan cupang yang bernilai ekonomis yaitu ikan cupang jantan, karena memiliki bentuk yang indah. Untuk meningkatkan produksi ikan jantan, langkah yang dapat dilakukan dengan cara maskulinisasi. Penelitian ini bertujuan untuk untuk mengetahui pengaruh ekstrak seledri terhadap persentase ikan cupang jantan dengan cara maskulinisasi. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 6 perlakuan dan 3 ulangan. Perlakuan dalam penelitian ini adalah larva ikan cupang umur 7 hari yang direndam dalam media ekstrak seledri dengan konsentrasi berbeda selama 8 jam. Perlakuannya adalah penambahan ekstrak seledri sebanyak 5 mg / L (P1), 10 mg / L (P2), 20 mg / L (P3), 40 mg / L (P4), 80 mg / L (P5) dan tanpa penambahan ekstrak seledri sebagai kontrol (P0). Berdasarkan uji fitokimia, ekstrak etanol seledri mengandung steroid, flavonoid, tannin dan fenol. Hasil penelitian menunjukkan bahwa penambahan ekstrak seledri dengan perbedaan konsentrasi berpengaruh signifikan terhadap persentase ikan cupang jantan, tetapi tidak ada pengaruh yang signifikan terhadap tingkat kelangsungan hidup.

AbstrakKalsium dan fosfor merupakan unsur yang penting yang dibutuhkan untuk perkembangan dan pertumbuhan. Tulang bandeng diketahui memiliki kandungan kalsium dan fosfor yang tinggi. Tujuan dari penelitian ini adalah mengetahui proksimat dan kandungan kalsium dan fosfor pada crackers yang difortifikasi dengan tepung tulang ikan bandeng. Fortifikasi crackers tepung tulang ikan bandeng menggunakan 4 perlakuan yakni presto, kukus, segar dan kontrol. Analisis proksimat meliputi kadar air, kadar abu, lemak, protein dan karbohidrat menggunakan metode AOAC (2005) dan analisis kandungan fosfor dan kalsium menggunakan metode AAS. Kandungan kalsium dan fosfor crakers tepung tulang masing-masing berkisar 88916-119730 mg/kg dan 2,2 mg/kg - 7,4 mg/kg. Nilai proksimat meliputi kadar air 12,26 – 14,42%, kadar abu 16,12 – 21,67%, protein 0,626 – 7,304%, lemak 4,0 – 4,8% dan karbohidrat 37,64 – 41,88%. Fortifikasi tepung tulang bandeng meningkatkan nilai proksimat, kalsium, dan fosfor pada crackersAbstractCalcium and phosphorus are important elements needed for development and growth. Milkfish is known to have high calcium and phosphorus content. The aim of study was to determine the proximate, and the content of calcium and phosphorus in crackers fortification with milkfish bone flour. Cracker fortification with milkfish bone flour consists of four treatments were presto, steamed, fresh and control. Proximate analysis includes water, ash, fat, protein and carbohydrates using the method (AOAC 2005) and analysis of phosphorus and calcium content using the AAS method. Calcium and phosphorus content bone flour crackers range respectively 88916-119730 mg/kg and 2.2 mg/kg – 7.4 mg/kg. Moisture content 12.26 – 14.42%, ash content 16.12 – 21.67%, protein 0.626- 7.304%, fat 4.0 – 4.8% and carbohydrates 37.64 – 41.88%. Fortification of milkfish flour increases the proximate, calcium, and phosphorus value in crackers.

HighlightsThe Paku Uban (Nephrolepis biserrata) was identified and analyzedThe ability of paku uban to stimulated molting process of Scylla sp was analyzedThe paku uban (Nephrolepis biserrata) could increase the presentation of molting and accelerate molting processAbstractMud crab (Scylla spp.) is a fishery commodity with high economic value. For this reason, efforts to increase production are required with a more effective applicative technology. This study aims to determine the molting response and survival rate of mud crabs injected with paku uban leaf  extract (N. biserrata) at different doses. The study was conducted for 30 days, located in Tibi Island, Tanah Lia District, Tanah Tidung. The design used in this study was a completely randomized design (CRD) with five treatments and eight replicates. Mud crabs weighing 80 - 150 g were tested in K1 (without injection), K2 (controlled injection), P1 (100 ppm extract), P2 (125 ppm extract) and P3 (150 ppm extract). The effectivity of paku uban (N. biserrata) extract with the highest percentage of molting of 50% took place at P3, 37.5% at P2, and 25% at P1. The lowest levels of molting percentage were at K1 and K2 by 0%. The fastest molting period was ten days at P2, and the longest was 29 days at P1. The highest weight growth reached 33.75 g at P3, followed by P2 and P1 with 31 and 18.75 g, respectively. The survival rate of mud crabs for all treatments reached 100%. The results of the analysis of variance indicated a significant bet effect (P < 0.05) on the molting and weight growth percentage. The BNT test suggested that a dose of 150 ppm was the optimum treatment.

The Covid-19 pandemic has had a major impact on society, such as job losses, rising staples, and economic hardship. Prices of food needs that continue to increase and are not affordable have resulted in food problems, one of which is protein. One of the high-quality protein is protein from fish. Fish as a fishery commodity that is relatively easy to cultivate on a large industrial scale and household scale for food self-sufficiency is catfish (Clarias sp). The purpose of this activity is to add insight and knowledge of the community about budikdamber for food self-sufficiency. The method of implementing community service activities in Kampung Six is carried out by counseling and training methods on Budikdamber. In this training activity, a bucket with 40 liters of water and 20 catfish seeds was used. Maintenance was carried out for 60 days with ad satiation feeding three times a day. Observations were made on growth, survival and water quality. The results of the activity found that 93% of the community partners had understood about Budikdamber. Budikdamber maintenance by partner communities gets an absolute weight growth value of catfish of 14 g, catfish survival value of 75%, and 1 bunch of kale per bucket. Based on the results of this activity, it is hoped that there will be the provision of special land for Budikdamber as an example for people who want to develop in their respective yards.

Fish farming has become a significant sector in producing animal protein. However, a disease caused by bacteria such as Aeromonas hydrophila and Vibrio parahaemolyticus has become obstacle factors in these farming activities. Among the various procedures for preventing bacterial attacks, the use of natural ingredients deserves to be considered. This study aimed to test the ability of gray nail extract (Nephrolepis biserrata) in inhibiting the growth of A. hydrophila and V. parahaemolyticus in vitro. N. biserrata were extracted using methanol (EPM) and chloroform (EPC). EPM and EPC were then challenged with A. hydrophila and V. parahaemolyticus bacteria to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The test results showed that an inhibition zone of EPM against A. hydrophila and V. parahaemolyticus were 8.2 and 8.3 mm, respectively. EPC has been unable to inhibit the activity of both bacteria, whereas EPM could. The MIC value for both bacteria was 50 mg/mL, and MIB concentration was more than 50 mg/mL. The results of this study indicate that the N. biserrata extract could inhibit bacteria.

Perairan pantai amal merupakan perairan terbuka yang mendapatkan pengaruh besar dari lingkungan sekitarnya. Penelitian dilakukan untuk mengetahui tingkat toksisitas perairan sungai Amal Lama dengan cara pengambilan sampel air dan biota yang diambil di Sungai Amal Lama Kota Tarakan. Pengujian kualitas air meliputi nitrit secara spektrofotometri, kadar amoniak secara fenat, sulfida total secara iodometri, serta analisis kandungan logam (Al, Mn, Zn, Fe dan Cu) dalam biota. Data yang di peroleh dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa kadar sulfida total, amoniak, dan nitrit dari sampel uji secara berurutan adalah 4 mg/L, 0,299 mg/L, dan nitrit 0,065 mg/L. Hasil uji logam Al, Mn, Zn, Fe, dan Cu nilai yang diperoleh adalah 0,07; 0,4; 0,1; 0,1 dan 0,6 mg/kg. berdasarkan hasil ini, dapat disimpulkan bahwa perairan Sungai Pantai Amal Lama masih layak untuk kehidupan organisme.