The extended-spectrum b-lactamase (ESBL) producer bacteria until now were mostly identified in hospital environment. The aim of this study was to analyze the prevalence of ESBL-producing gut flora and distribution of ESBL encoding genes between hospitalized patient in Tropical Wards of Dr. Soetomo Hospital and patient from a primary health centre (PHC) as community environment in Surabaya. Thiry rectal swab samples from hospital of Dr. Soetomo patients and from PHC (60 samples in total) were collected for this study. Samples were screened in MacConkey agar supplemented with 2 mg/L of cefotaxim, incubated at 37ºC for 24 hours. Then the growing colony were confirmed with Disk Diffusion Synergy test (DDST) for diagnosis of ESBL producer. The identified ESBL producers were then identified the bacteria species by biochemical method. ESBL gene were detected by PCR with specific primers. The results showed that there was not difference of positif nuber of ESBL-producing bacteria gut floral between patients of Dr.Soetomo Hospital, 25/30 (83.3%) and PHC, 11/30 (36.7%) (p=1). The pattern of ESBL gene distributions among samples from hospital showed that SHV was 12%, TEM was 36%, and CTX-M was 80%, and from PHC were SHV 18.2%, TEM 27,3% and CTX-M 81,8%. Statistical analysis showed that the pattern was not significantly different among hospitals and PHC samples as shown by SHV gene (p=0,631), TEM (p= 0.715), and CTX -M (p=1). From each ESBL gene, the dominant genes that found producing ESBL were the CTX-M genes followed by TEM and SHV genes. The prevalence of ESBL producersin intestinal flora of both the hospital (83,3%) and the PHC (36,7%) was very high. There was not significant difference between the prevalence of ESBL producer in gut flora of hospitalized patients compared to PHC. There was found other patterns of ESBL gene combinations in the hospital of SHV+CTX-M genes, TEM+CTX-M, SHV+TEM+CTX-M genes and PHC, the combination pattern of SHV+CTX-M, TEM+CTX-M.
Kekurangan gizi pada bayi dan anak akan menimbulkan gangguan pertumbuhan dan perkembangan apabila tidak diatasi secara dini dapat berlanjut hingga dewasa. Penanggulangan balita gizi kurang dilakukan dengan pemberian makanan tambahan (PMT). Kebaruan penelitian ini karena meneliti tentang efektivitas pelatihan pembuatan pmt berbasis pangan lokal dalam meningkatkan pengetahuan ibu balita dalam mencegah stunting di wilayah kerja puskesmas tanjung karang. penelitian ini bertujuan untuk mengetahui pengaruh efektivitas pelatihan pembuatan PMT berbasis pangan lokal (Tempe dan Kelor) dalam meningkatkan pengetahuan ibu balita dalam mencegah stunting di wilayah kerja Puskesmas Tanjung Karang. Metode penelitian menggunakan quasi experiment dengan non equivalent control group design. Sampel penelitian sebanyak 27 responden pada masing-masing kelompok kontrol dan kelompok perlakuan. Analisis data menggunakan Wilcoxon test. Hasil penelitian menunjukkan bahwa terjadi peningkatan pengetahuan responden pada kelompok perlakuan setelah diberikan pelatihan pembuatan PMT dengan kategori baik sejumlah 1 (3.7%) meningkat menjadi 13 (48.1%). Hasil penelitian juga menunjukkan efektivitas pelatihan pembuatan PMT berbasis pangan lokal berpengaruh terhadap tingkat pengetahuan pada kelompok perlakuan p=0.000 (p0.05) dan tidak ada perbedaaan pada kelompok kontrol p=0.655 (p0.05). Kesimpulan, adanya peningkatan pengetahuan ibu balita setelah diberikan pre-test dan post-test efektivitas pelatihan pembuatan PMT berbasis pangan lokal dalam mencegah stunting.Kata kunci: Ibu Balita; PMT; Pelatihan; Pengetahuan; Stunting.Abstract Malnutrition in infants and children will cause growth and development disorders, if not treated early, can continue into adulthood. Management of undernourished children under five is done by providing additional food (PMT). The novelty of this study is because it examines the effectiveness of training on making local food-based PMT in increasing the knowledge of mothers of toddlers in preventing stunting in the work area of the Tanjung Karang Health Center. This study aims to determine the effect of the effectiveness of training on making local food-based PMT in increasing the knowledge of mothers under five in preventing stunting in the Tanjung Karang Public Health working area. The research method uses a quasi-experimental with non-equivalent control group design. The research sample was 27 respondents in each control group and treatment group. Data analysis using Wilcoxon test. The results showed that there was an increase in respondents' knowledge in the treatment group after being given training on making PMT with a good category of 1 (3.7%) increasing to 13 (48.1%). The results also showed that the effectiveness of training on making PMT based on local food had an effect on the level of knowledge in the treatment group p = 0.000 (p 0.05) and there was no difference in the control group p = 0.655 (p 0.05). In conclusion, there was an increase in the knowledge of mothers of toddlers after being given pre-tests and post-tests on the effectiveness of training on making local food-based PMT in preventing stunting.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.