The Prevalence of Extended Spectrum Beta-Lactamase (ESBL) Producing Gut Bacterial Flora Among Patients in Dr. Soetomo Hospital and Primary Health Centre in Surabaya

Naelasari, Dian Neni; Koendhori, Eko Budi; Dewanti, Linda; Sarassari, Rosantia; Kuntaman, Kuntaman

doi:10.20473/fmi.v54i4.10708

Cited by 5 publications

(7 citation statements)

References 25 publications

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“…The gene type of bla TEM has been reported to be responsible for 90% of ampicillin resistance in E. coli, while this study also showed that the isolates were resistant to ampicillin with 50% (15/30) detected bla TEM [18]. Multiple genes patterns of bla TEM and bla CTX-M were predominantly found in this study, which is consistent with previous studies in Surabaya, conducted by Naelasari et al (24%) and in Denpasar, conducted by Fatmawati et al (15.5%) [19,20] This pattern was also consistent with another study in Iraq, reported by Pose et al that bla TEM and bla CTX-M were dominant (30.9%). Such molecular patterns also showed similar antibiotic sensitivity patterns, which were mostly sensitive to meropenem and amikacin [21].…”

Section: Discussionsupporting

confidence: 91%

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Characteristic of Extended Spectrum β-Lactamase-Producing Enterobacteriaceae from Fecal Carriage Isolates of Intensive Care Unit Patients at Sanglah Hospital, Bali, Indonesia

Candra¹,

Yanto²,

Suranadi³

et al. 2021

TOMICROJ

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Background: The increasing Extended-Spectrum β-Lactamases-producing Enterobacteriaceae (ESBL-PE) infections in the Intensive Care Unit (ICU) needs an early warning system for the detection of these bacteria. The ESBL-PE fecal carriage analysis is a screening method that can be used to detect and characterize these bacteria. Furthermore, it aids in assessing an ICU patient’s risk of possible infection and prevent its transmission to the other patients within the period of hospitalization; therefore, enhancing the quality of patient care while alsoreducing morbidity and mortality due to ESBL-PE infection in ICU. Objective: The study aimed to determine the antibiogram and molecular characteristics of ESBL-PE fecal carriage from ICU patients at Sanglah Hospital, Denpasar, Bali. Methods: This cross-sectional retrospective study involved 30 stored-bacterial isolates of ESBL-PE from a rectal swab of ICU patients who had just been admitted to the ICU of Sanglah General Hospital from February to March 2019, consecutively. The identification and antimicrobial susceptibility test of the isolates were conducted using Vitek-2 Compact (bioMérieux®, Marcy-l'Etoile, France), while genotype identification was conducted using PCR for the detection of blaTEM, blaSHV, blaCTX-M genes. Results: Thirty bacterial isolates were identified as Escherichia coli (24/30) and Klebsiella spp. (6/30) and detected as ESBL-producing isolates by Vitek-2 Compact. All isolates were susceptible to piperacillin-tazobactam, meropenem, and amikacin. Twenty-two (73.3%) isolates harbored ESBLs blaTEM, blaSHV, blaCTX-M genes, either individually or in combination. Most of the isolates had the combination of ESBL genes. About 20% (6/30) of isolates had a combination of blaTEM and blaCTX-M, while 10% (3/30) of them possessed all of the three genes detected in this study. Only 3.3% (1/30) of the isolates had each combination of blaTEM and blaSHV as well as blaSHV and blaCTX-M. Meanwhile, 16.7% (5/30) of the isolates were detected to have each single gene of blaCTX-M or blaTEM, and only one isolate (3.3%) harbored blaSHV. Conclusion: High prevalence of blaTEM, blaSHV, and blaCTX-M ESBL genes harbored by fecal flora of patients who had just been admitted in ICU give rise to the risk for transmission among critically ill patients in ICU. Fecal screening of ESBL-PE besides infection control can be considered for those patients who have a risk factor of ESBL-PE colonization before they are being admitted to the ICU.

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Section: Discussionsupporting

confidence: 91%

“…The prevalence of ESBLs type, bla CTX-M was still more dominant than other types in Surabaya that reported 80% and 94.5% in E. coli and 55.6% in K. pneumonia [19,22]. Other studies in Singapore, Vietnam, and Thailand also showed a prominent spreading of CTX-M type ESBLs.…”

Section: Discussionmentioning

confidence: 97%

Characteristic of Extended Spectrum β-Lactamase-Producing Enterobacteriaceae from Fecal Carriage Isolates of Intensive Care Unit Patients at Sanglah Hospital, Bali, Indonesia

Candra¹,

Yanto²,

Suranadi³

et al. 2021

TOMICROJ

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“…ESBL ESBL is produced by gram-negative bacteria, for example the family Enterobacteriaceae which is a normal intestinal flora where this bacterium has been resistant to beta lactam antibiotics and is one of the main causes of bacterial infections in hospitals or in the community. 16…”

Section: Resultsmentioning

confidence: 99%

Antibiotic sensitivity on pathogenic bacteria causing bacterial vaginosis

Linggarjati

Parti

Sakinah

2021

MOG

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Objectives: To identify the sensitivity of antibiotics to pathogenic bacteria that cause Bacterial Vaginosis (BV).Materials and Methods: This type of research was an observational study with a sample of six specimens. The data were taken using primary data from patients who were swabbed in the vagina and then diagnosed BV with amsel criteria on vaginal secretion specimens carried out at Tanggul health center on January 23-February 23, 2020. The specimens were sent to Parahita Clinical Laboratory for bacterial identification and adjusted for sensitivity with CLSI using vitek 2 compact tool.Results: The results of this study identified the bacteria that caused bacterial vaginosis, the E. coli and K. pneumoniae with one sample of suspected ESBL. ESBL is a beta lactamase enzyme produced by bacteria and can induce bacterial resistance to penicillin, cephalosporin generation 1, 2, and 3. The types of bacteria found were E. coli and K. pneumoniae with high sensitivity antibiotics tested including piperacillin/tazobactam, ceftazidime, cefepime, ertapenem, meropenem, amikacin, gentamicin, tigecycline, and nitrofurantoin. Antibiotics with high levels of resistance tested against these bacteria included: ampicillin, amoxicillin, and ampicillin/sulbactam due to the mechanism of beta-lactam antibiotic resistance in the production of beta lactamase from bacteria.Conclusion: The type of bacteria found was E. coli and K. pneumoniae with high resistance levels in beta lactam antibiotics.

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“…ESBL-producing bacteria were confirmed using DDST (Double Disk Synergy Test) using antibiotic disc of ceftriaxon, cefotaxim, cefepim and aztreonam with amoxicillin-clavulanic acid at the center of the plate. (9) The identification was performed at Microbiology Laboratory, Dr. Soetomo General Hospital Surabaya. Data analysis was conducted using SPSS Statistical Software version 20.…”

Section: Methodsmentioning

confidence: 99%

Prevalence ESBL Producing Escherichia Coli among Children in Indonesia

Happy¹,

Setyarini²,

Ranuh³

et al. 2020

IJPHRD

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Introduction: Beta-lactam antimicrobials are the most often antibiotic used for the treatment of infections worldwide, including Indonesia. Continuous exposure of these antimicrobials will induce the increasing of antimicrobial resistance, such as extended spectrum β-lactamase (ESBL). The carrier of ESBL among people will be serve as a risk factor to get an infection, especially among low immune response hospitalized patients. The aims of this study was to explore the ESBL producing gut flora and carrier rate among children visiting Primary Health Center (PHC) in Surabaya. The cross sectional study was conducted in three PHCs since February 2019 until April 2019. The total 200 children were swabbed from rectal, 100 from neonatal age and 100 from post neonatal age, then sub-cultured in MacConkey medium supplemented with cefotaxime 2 micrograms per milliliter. The growth colonies were then confirmed by DDST (Double Disk Synergy test) for ESBL producer and biochemical test for bacterial diagnosis. Result: Among 100 neonatal age were identified 14 (14%) producing ESBL consist of 9 (64,3%) ESBL producing Escherichia coli,5 (35,7%) ESBL producing non Escherichia coli. Whether among 100 post neonatal age were 37 (37%) producing ESBL consist of 25 (67,6%) ESBL producing Escherichia coli and 12 (32,4%) producing ESBL non Escherichia coli. Conclusion:Prevalence ESBL producing Escherichia coli on post neonatal age was significantly different than neonatal age (p = 0,001). Risk Factors of ESBL producing Escherichia coli on Post neonatal age and neonatal age are history of visiting hospital (p = 0,026), history of antibiotics used by mother (p = 0,043), mode of delivery (p = 0,032

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The Prevalence of Extended Spectrum Beta-Lactamase (ESBL) Producing Gut Bacterial Flora Among Patients in Dr. Soetomo Hospital and Primary Health Centre in Surabaya

Cited by 5 publications

References 25 publications

Characteristic of Extended Spectrum β-Lactamase-Producing Enterobacteriaceae from Fecal Carriage Isolates of Intensive Care Unit Patients at Sanglah Hospital, Bali, Indonesia

Characteristic of Extended Spectrum β-Lactamase-Producing Enterobacteriaceae from Fecal Carriage Isolates of Intensive Care Unit Patients at Sanglah Hospital, Bali, Indonesia

Antibiotic sensitivity on pathogenic bacteria causing bacterial vaginosis

Prevalence ESBL Producing Escherichia Coli among Children in Indonesia

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