Original Research ArticleThe population of Sunda porcupine (Hystrix javanica) is tends to decrease year by year, since it's become rarely found in nature. This study was conducted to determine the histological structure and distribution of estrogen and progesterone receptors in the ovary of pregnant and non-pregnant Sunda porcupine. Five ovaries of pregnant and nonpregnant Sunda porcupine were used as samples. The ovaries were fixed in Bouin's solution, processed with paraffin method, and cut into 5μm thickness. The tissue slides were dyed using immunohistochemical staining method to determine the distribution of estrogen and progesterone receptors. The results were analyzed descriptively. Immunoreactivities of estrogen and progesterone receptors in the ovaries of non-pregnant Sunda porcupines were detected in the atretic follicle cells and theca internal cells of developing follicle, whereas in the ovaries of pregnant Sunda porcupine, immunoreactivities of estrogen and progesterone receptors only found in atretic follicle cells. Luteal cells of corpus luteum appeared to be immunoreactive against progesterone receptor antibodies with weak intensity in the ovary of pregnant Sunda porcupine. This study was confirming that there was little difference in expression of estrogen and progesterone receptors in ovaries of non-pregnant and pregnant Sunda porcupine.
The population of Sunda porcupine (Hystrix javanica) declines each year since it is rarely found in nature. The present study aimed to obtain information about the distribution of carbohydrate residues contained in immature and mature of Sunda porcupine's testes and to discuss its relevant functions. This study used six testes obtained from four immature and two mature Sunda porcupine originated from Ngawi Regency, East Java Province, Indonesia. Testis tissues were stained with hematoxylin and eosin and lectin histochemistry of Lens culinaris agglutinin (LCA), Phaseolus vulgaris leucoagglutinin (PHA-L), Pisum sativum agglutinin (PSA), Sophora japonica agglutinin (SJA), and Wheat germ agglutinin (WGA). Data were analyzed with descriptive and semi-quantitative method. Lectin histochemical staining with LCA, PHA-L, SJA, PSA, and WGA indicated the presence of alpha-D-mannose and alpha-D-glucose, N-acetylgalactosamine, mannose, and N-acetylglucosamine residues in the immature and mature testes with weak to very strong intensity. In the immature testes of Sunda porcupine, there was positive reactivity with PHA-L for Leydig and Sertoli cells, N-acetylgalactosamine may play an important role in the development and maturation of Leydig and Sertoli cells. Mature testes showed a strong positive reaction to the LCA, SJA, PSA, and WGA which indicated the significant roles of alpha-D-mannose and alpha-D-glucose, N-acetylgalactosamine, mannose, and N-acetylglucosamine residues on the maturation process of early spermatid to the late spermatid. These results can be used as basic data to be implemented in the conservation efforts of Sunda porcupine.
Sunda porcupines (Hystrix javanica) belongs to the Order of Rodentia is an endemic animal of Indonesia. The aims of this study are to determine the types and histological structure of Sunda porcupine placenta. Data regarding the type and histological structure of Sunda porcupines' placental organs can be used to support the conservation of Sunda porcupines. This research used two samples of placenta Sunda Porcupine from Ngawi, East Java. Placenta were fixed in Bouin's solution for 24 hours. Tissues were processed by using paraffin method and cut in 5 µm thickness. Tissues slide were stained with Hematoxylin Eosin (HE) to identified histological structure of the Sunda porcupine placenta. Photomicrographs were using Optilab Image Viewer. The histological structure of the Sunda porcupine's placenta were analyzed and reported descriptively. Macroscopically, the shape of Sunda Porcupine placenta is flat like a disc. Histologically, the thickest parts of Sunda porcupines placenta consist of chorioallantoic plate, labyrinth zone, trophospongium, decidua, metrial glands, myometrium zone. In conclussion, the placenta of sunda porcupine (Hystrix javanica) has been identified which has a discoid shape and is classified to the hemochorial type.
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