Tooth hypersensitivity is a clinical condition that describes localized, non spontaneous pain, and arises when tooth is stimulated using thermal, chemical, and mechanical stimuli. The use of desensitizing agent such as hydroxyapatite and Eugenia caryophyllus can be used as home treatment that can relieve tooth hypersensitivity. Hydroxyapatite is an inorganic compound that makes up the hard tissues of the human body such as bone, dentine and tooth. Hydroxyapatite has a mechanism to decrease dental hypersensitivity by inducing remineralization of the teeth and close the open dentinal tubules. Eugenia caryophyllus is a material that is used in the field of dentistry and can reduce the pain of tooth hypersensitivity by suppressing nerve receptors in the perception of pain felt by the patient. The aim of this study is to determine  the influence of hydroxyapatite-based toothpaste and Eugenia caryophyllus against tooth hypersensitivity. In this observational research, researcher give different type of toothpaste to two groups of research subjects and measure the changes in tooth sensitivity for 15 days. The value of tooth hypersensitivity before and after treatment is tested using Electric Pulp Test (EPT).  Independent t-test shows the result of p value = 1.30 (p>0.05). It can be concluded that there is no significant difference between hydroxyapatite and Eugenia caryophyllus in relieving tooth hypersensitivity after being used for 15 days.

BACKGROUND: Bromelain is a sulfhydryl proteolytic enzyme that can hydrolyze protein, protease or peptide. Bromelain can be found in pineapple stem, fruit and core. Bromelain is composed of 212 amino acid residues with cysteine-25 forming a polypeptide chain that can hydrolyze peptide bonds by H2O. In medicine, bromelain has been developed as antibiotic, cancer drug, anti-inflammatory agent and immunomodulator. In dentistry, bromelain has potential to reduce plaque formation on the teeth and to irrigate root canal.METHODS: Pineapple core was dried for 3 days to get simplicia. Then simplicia was extracted with water solvent for 24 hours. After that, the macerated-pineapple core crude extract-derived bromelain (PCB) was separated by Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) followed by Coomassie Brilliant Blue (CBB) staining to ensure the presence of bromelain. In cytotoxic test, NIH-3T3 fibroblast cultures were treated with extracts in various concentrations to for 24 or 48 hours. Number of fibroblasts was calculated using 3-(4,5-dimethylthiazol-2- yl)-2,5-Diphenyltetrazolium bromide (MTT) assay.RESULTS: Pineapple core extraction using maceration method produced relative high yield (concentration: 1.5424 g/mL) of bromelain, which was confirmed by CBB staining results with the molecular weight of 33 kDa. Based on cytotoxic test results of PCB on NIH-3T3 fibroblasts, 24-hours-incubation LD50 was 95.7 g/L, while 48-hours-incubation LD50 was 51.1 g/L.CONCLUSION: PCB has low cytotoxic effect in NIH-3T3 fibroblasts.KEYWORDS: bromelain, pineapple, extract, cytotoxic, MTT

The problem of domestic waste in Indonesia related to the behavior of people who do not understand the impact of poor environmental hygiene which will lead to the emergence of various diseases in the body and oral cavity. Domestic waste is the remaining waste generated from household activities. Domestic waste is divided into organic that can be processed and inorganic that cannot be processed. The most pronounced impact is the emergence of various diseases such as intestinal worms, diarrhea, cholera, typhoid and anemia while infection can occur in the oral cavity so that infection can occur locally or systemically. Community Service (PkM) was carried out offline at the Grogol Petamburan Hall which was attended by 37 representatives of dasawisma and jumantik cadres, heads of RT 01 – 14 and RW 01. The material provided was in the form of various kinds of domestic waste (organic and inorganic), management of domestic waste, diseases that arise in the body and oral cavity due to a dirty environment, how to wash hands and how to brush your teeth properly in the form of ppt, e-flyer and video. Evaluation of success by conducting a pre and post test containing 10 knowledge questions and 10 attitude questions. There was an increase in participants knowledge of 22.3% and an improvement in attitude towards a better one. The results obtained from this activity are very good with partners willing to carry out PkM activities. At the end of the activity, toothpaste and a donation of trash bins were given to each RT.

<p><strong><em>Background</em></strong><em>: </em><em>Many people are very concerned about performance especially in terms of the appearance of their teeth. Various attempts were made to improve the appearance of teeth by bleaching. Carbamide peroxide and hydrogen peroxide as bleaching materials may release free radicals which affect surface roughness of the composite restoration. <strong>Objective</strong>: </em><em>to determine the effect of carbamide peroxide 20% and 35% on micro hybrid resin composite</em><em> </em><em>hardness</em><em> </em><strong><em>Methods: </em></strong><em>Samples are micro hybrid resin composite with 4 mm thickness and 10 mm diameter. Total number is 10 samples for each groups which were divided into 2 groups: group 1 application of 20% carbamide peroxide and group 2 application of 35% carbamide peroxide. Each group were tested prior, after 7 and 14 days using Vickers Hardness. <strong>Result: </strong>The results indicate that there was no significant effect of 20% carbamide peroxide on resin composite hardness for 7 days with (p &gt; 0.05) and 35% for 7 days (p &gt; 0.05), but the use of carbamide peroxide 20% and 35% on a micro hybrid resin composite for 14 days yielded a significant increase in hardness (p&lt;0.01)<strong>. Conclusion: </strong>The use of carbamide peroxide 20% and 35% for 7 and 14 days did not cause hardness decrease on micro hybrid resin composite.</em></p>

<table border="0" cellpadding="0"><tbody><tr><td valign="top"><p><strong><em>Background:</em></strong><em> </em><em>Bulk-fill composite resin is a tooth-colored restoration material that is developed to obtain better aesthetic and mechanical properties, which include compressive strength. The factors influencing the reduction of compressive strength of composite resin are hydrophilic matrix monomers and inadequate bonds between compositions. One of the absorbable liquids is a mouthwash commonly used to control dental caries and reduce the amount of plaque after brushing the teeth. <strong>Objective:</strong> To determine the effect of mouthwash containing 9% alcohol and nonalcohol on the compressive strength of bulk-fill composite resin. <strong>Method:</strong> The research type conducted is an experimental laboratory. The compressive strength of bulk-fill composite resin will be tested using Universal Testing Machine. There were three treatment groups, which are immersion in mouthwash containing 9% alcohol, nonalcohol, and artificial saliva as a control group. Samples were immersed at room temperature 25°C for 12 hours according to instructions for use twice a day for 30 seconds. <strong>Results:</strong> The One Way ANOVA test shows a significant value, which was sig. 0.001 (p &lt; 0.05). There were significant differences in the test of the effect of mouthwash containing 9% alcohol and nonalcohol mouthwash on the compressive strength of bulk-fill composite resin. This result is caused by the hydrophilic traits of the matrix monomers, mechanisms that occur in resin compositions, acidity and alcohol content of mouthwash can affect the reduction in compressive strength of the composite resin. <strong>Conclusion:</strong> The mouthwash containing 9% alcohol reduced the compressive strength of bulk-fill composite resin more compared to nonalcohol mouthwash</em><em>.  </em></p></td></tr></tbody></table>