No abstract

The main goal was directed to better understanding of the intra- and extra-cellular inter-molecular interactions as a part of the regulatory mechanisms, by which different cells, tissues and organs communicate to each other. The functions of protein Secretagogin (SCGN) and tri-peptide Glutathione (GSH) as tumor suppressors, neuroprotectors and endocrine regulators are known. The participation of these molecules in the regulation of cellular growth, proliferation, differentiation and death, but also of inflammatory and pro-inflammatory processes has also been proved. The role of SCGN and GSH in brain and pancreas of rat origin was investigated in the current study. Total lysates from rat brain and pancreas were prepared (controls). Equal volumes were passed through GSH-agarose columns to select molecules with affinity to GSH. Separate aliquotes were mixed with a total lysate of laboratory-incubated RIN-5F malignant rat insulinoma cells, which contain an additional copy of the tumor-suppressor gene scgn, coding the protein Secretagogin (SCGN), inserted by transfection with appropriate recombinant DNA-vectors, containing also a GST-tag. The average titers of gangliosides and of anti-ganglioside antibodies were determined by enzyme-linked immunosorbent assay (ELISA). The possibility for production of immunoglobulins/antibodies by non-lymphoid cells, tissues and organs was proposed, probably as one of the steps in the cascade regulatory mechanisms. Because the produced antibodies are outside the germinative centers of the specialized lymphoid tissues and organs, control of their function by small molecules as gangliosides is very important, acting directly or indirectly on different intra- and extra-cellular inter-molecular interactions in various cascade regulatory pathways.

The main goal was related with a better understanding of the intra- and extra-cellular inter-molecular interactions in the different regulatory pathways, by which the separate cells, tissues and organs communicate to each other. The functions of tri-peptide Glutathione (GSH) as tumor suppressor, neuroprotector and endocrine regulatos, but also its role in the regulation of cellular growth, proliferation, differentiation and death, as well as of inflammatory and pro-inflammatory processes has been proved. In the current study, total extracts (controls) from rat brain and rat pancreas were prepared. Separate amounts were passed through GSH-agarose columns to select molecules, possessing affinity to GSH. The average titers of gangliosides and of anti-ganglioside antibodies in the so prepared biological samples were determined by enzyme-linked immunosorbent assay (ELISA). Possibility about production of immunoglobulins/antibodies by non-lymphoid cells, tissues and organs was suggested, probably as one of the steps in the regulatory pathways. Because the so produced antibodies are outside the germinative centers of the specialized lymphoid tissues and organs, control of their function by small ions and molecules as gangliosides is very important, by direct intra- and extra-cellular inter-molecular interactions or by indirect influence on various regulatory mechanisms.

Initial myeloid and lymphoid differentiation of hematopoietic and non-hematopoietic stem/progenitor cells by appropriate ex vivo-and in vitro-incubation was evaluated. So, hematopoietic stem/progenitor cells from mouse spleen were ex vivo-incubated in the presence of different interleukins, as well as of various combinations of them. Non-hematopoietic mouse embryonic stem cells (mESCs) were in vitroincubated in the presence of GM-CSF and malignant antigens of human cervical carcinoma cells HeLa. Analogically, mouse embryonic fibroblasts from line 3T3 were pre-incubated in the presence of malignant antigens from mouse myeloma cells. Possibilities for derivation of myeloid and lymphoid cells by hematopoietic and non-hematopoietic cellular progenitors were proposed. The literature ability for production of immune molecules, including membrane glycoprotein receptors and antibodies/immunoglobulins by initial myeloid and lymphoid cells, derived from hematopoietic, as well as by non-hematopoietic cellular progenitors was accepted when appropriate factors are available. Because the produced antibodies are out of the germinative centers of the specialized lymphoid tissues and organs, control of their function is very important, for escape of chronic inflammatory processes. Realistically, this control proved the role of small ions and molecules, by direct and/or indirect influence on different intra-and extra-cellular inter-molecular interactions in various cascade regulatory pathways.