Spirulina platensis is a unicellular microalgae that has high nutritional value such as proteins, carbohydrates, lipids and carotenoids like β-carotene. The microalgae rapidly grows in Bold Bassal Medium modification and high intensity of light. The aims of this research were to optimize the culture growth and determine antioxidant activity of S. platensis. Culture was optimized by adding urea and NaHCO3 I nto the medium and applying dark : light period (12:12 h/h) under solar or fluorescent light (3000 lux). The optimum growth of Spirulina platensis was in 0,3 g/L urea, pH 9 at fluoresense light (3000 lux). Antioxidant acivity of various spirulina extract was studied by 1,1diphenyl-2-picryl-hydrazyl (DPPH) free radicals scavenging method. The Spirulina radicals scavenging activity of acetone : methanol (1:1) extract was 46,39 %. The acetone:methanol (1:1) extract showed better antioxidant activity than acetone extract. The β-carotene was determined using HPLC in a silica gel column with dichloromethane, acetonitrile, methanol (20:70:10, v/v/v) as a mobile phase. The acetone extract displayed the highest sample area and produced 1.32% of β-carotene out of total biomass.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.