Spirulina platensis is a unicellular microalgae that has high nutritional value such as proteins, carbohydrates, lipids and carotenoids like β-carotene. The microalgae rapidly grows in Bold Bassal Medium modification and high intensity of light. The aims of this research were to optimize the culture growth and determine antioxidant activity of S. platensis. Culture was optimized by adding urea and NaHCO3 I nto the medium and applying dark : light period (12:12 h/h) under solar or fluorescent light (3000 lux). The optimum growth of Spirulina platensis was in 0,3 g/L urea, pH 9 at fluoresense light (3000 lux). Antioxidant acivity of various spirulina extract was studied by 1,1diphenyl-2-picryl-hydrazyl (DPPH) free radicals scavenging method. The Spirulina radicals scavenging activity of acetone : methanol (1:1) extract was 46,39 %. The acetone:methanol (1:1) extract showed better antioxidant activity than acetone extract. The β-carotene was determined using HPLC in a silica gel column with dichloromethane, acetonitrile, methanol (20:70:10, v/v/v) as a mobile phase. The acetone extract displayed the highest sample area and produced 1.32% of β-carotene out of total biomass.
Microalgae are photosynthetic prokaryotic and eukaryotic microorganisms. In this research, isolation of microalgae from Maninjau lake West Sumatra was identified by morphological and molecular identification by PCR using primers for 16S rDNA and 18S rDNA prokaryotic and eukaryotic microalgae. The results of this study were obtained 3 isolates of microalgae that can be isolated from Maninjau lake West Sumatra are Scenedesmus (code isolate AUMA-020) with a percent similarity of 95%, Uncultured cyanobacterium (code isolates AUMA-023) with a percent similarity of 80% and Limnothrix (code isolates AUMA-019) has a percent similarity of 98% based on data gene Bank blast results.
Waste activated sludge of crumb rubber industry (CRI-WAS) is discarded as waste from wastewater treatment processing. Therefore it is necessary to develop and innovate to convert the material becomes valuables and expected to be an economical adsorbent for metal ion remediation from wastewater. The ability of three types of adsorbents prepared from CRI-WAS for the removal of Zn(II) from aqueous solutions has been investigated. The XRF, BET, SEM-EDX, and FTIR analysis were conducted to characterize the PS (pure sludge), WS (washed sludge) and P600 (pyrolysis at 600 0 C) CRI-WAS types of adsorbents. In the batch system, experimental parameters were investigated, including solution pH, contact time, and adsorbent dose. The data were analyzed by analysis of variance (ANOVA) and a further test Duncan's New Multiple Range Test (DNMRT) at the 5% significance level. The results reveal that all types of adsorbent exhibited good sorption potential at pH 5.0. The P600 has largest adsorption capacity. The maximum adsorption capacity of the adsorbent for Zn(II) was found to be 10.276 mg g-1 , 11.042 mg g-1 and 64.169 mg g-1 for PS, WS and P600 CRI-WAS types of adsorbents respectively. The results showed that the adsorption isotherm data were fitted well by the Langmuir isotherm.
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