Abstract:One of the prior current astrobiological tasks is revealing the limits of microbial resistance to extraterrestrial conditions. Much attention is paid to ionizing radiation, since it can prevent the preservation and spread of life outside the Earth. The aim of this research was to study the impact of accelerated electrons (~1 MeV) as component of space radiation on microbial communities in their natural habitat-the arid soil and ancient permafrost, and also on the pure bacterial cultures that were isolated from these ecotopes. The irradiation was carried out at low pressure (~0.01 Torr) and low temperature (−130 • C) to simulate the conditions of Mars or outer space. High doses of 10 kGy and 100 kGy were used to assess the effect of dose accumulation in inactive and hypometabolic cells, depending on environmental conditions under long-term irradiation estimated on a geological time scale. It was shown that irradiation with accelerated electrons in the applied doses did not sterilize native samples from Earth extreme habitats. The data obtained suggests that viable Earth-like microorganisms can be preserved in the anabiotic state for at least 1.3 and 20 million years in the regolith of modern Mars in the shallow subsurface layer and at a 5 m depth, respectively. In addition, the results of the study indicate the possibility of maintaining terrestrial like life in the ice of Europa at a 10 cm depth for at least~170 years or for at least 400 thousand years in open space within meteorites. It is established that bacteria in natural habitat has a much higher resistance to in situ irradiation with accelerated electrons when compared to their stability in pure isolated cultures. Thanks to the protective properties of the heterophase environment and the interaction between microbial populations even radiosensitive microorganisms as members of the native microbial communities are able to withstand very high doses of ionizing radiation.

We briefly present preliminary results of our study of the radioresistant bacteria in a low temperature and pressure and high-radiation environment and hypothesize the ability of microorganisms to survive extraterrestrial high-radiation environments, such as the icy surface of Jupiter’s moon, Europa. In this study, samples containing a strain of Deinococcus radiodurans VKM B-1422T embedded into a simulated version of Europa’s ice were put under extreme environmental (−130 °C, 0.01 mbar) and radiation conditions using a specially designed experimental vacuum chamber. The samples were irradiated with 5, 10, 50, and 100 kGy doses and subsequently studied for residual viable cells. We estimate the limit of the accumulated dose that viable cells in those conditions could withstand at 50 kGy. Combining our numerical modelling of the accumulated dose in ice with observations of water eruption events on Europa, we hypothesize that in the case of such events, it is possible that putative extraterrestrial organisms might retain viability in a dormant state for up to 10,000 years, and could be sampled and studied by future probe missions.

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Methane seasonal variation observable by MSL mission and possible variations of atmospheric mass on timescale (105-106 years) are among the most intriguing problems in Mars exploration. These variations are connected with hypothetical biosphere activity in the subsurface Martian soil and existence of liquid water on the Martian surface within modern era. Stability of liquid water on surface request higher atmospheric pressure in comparing to modern value. CO2 cannot loss with known mechanisms of atmospheric escape. Therefore, the main part of necessary CO2 must be buried in upper layers of the Martian soil. Local and seasonal time variable sources and fast methane destruction are needed to explain high seasonal variations of methane concentration in air at the Martian surface. Gas reservoirs, containing biogenic or abiogenic methane could be possible seasonal sources of methane as well. In this work we experimental study stability of the gas reservoirs covered of mixture of regolith and water ice with perchlorates. Thickness of covered regolith layer was about 10mm. In experimental runs we increased a temperature of gas traps and monitored a possible diffusion of gases through the isolated layer with mass spectrometer. The gas traps stay stable at gas pressure up to 1 bar. We did not discover any diffusion process before mechanical destruction of reservoirs at gas pressure over 1 bar. In this work we show that the big subsurface gas reservoirs can exist for a long time before cracking due to slow process of the water ice sublimation by climate and seasonal variation of subsurface temperature.

The effect of radical accumulation in ice under high energy particle irradiation at low temperature followed by the energy release during heating has been proposed as a possible cause of cometary outbursts. Water ice samples were subjected to 15 MeV protons to simulate the cosmic ray irradiation of comet nuclei. The irradiation with 0.9 MeV electrons was performed to model a similar process on icy satellites located in radiation belts of planets. We observed the release of accumulated energy 20 J/g during the heating of the proton-irradiated ice with two pulses of fast self-heating of the sample at 84 and 110 K. Similar energy release occurred between 82 and 110 K in the electron-irradiated ice. Our calculations suggest that the recombination of radicals accumulated in the surface layer of a comet nucleus under cosmic ray bombardment can trigger the cometary outbursts far from the Sun.