Resistance of Enzymes to Ionizing Radiation under Model Conditions of the Martian Regolith

Cheptsov, V. S.; Belov, A. A.; Vorobyova, E. A.; Pavlov, A. K.; Tsurkov, Denis; Frolov, D. A.; Lomasov, V. N.; Chumikov, A. E.

doi:10.1134/s003809462104002x

Cited by 3 publications

(2 citation statements)

References 44 publications

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“…The effects of ionizing radiation also depend on exposure conditions. For instance, enzymatic activities were detected when irradiating with 100 kGy of g-rays at low pressure and low temperature (Cheptsov et al, 2021). In the absence of minerals, 37 kGy of g-rays caused an 85% degradation of purine and uracil, whereas Ertem et al (2021) observed 10% degradation under 5 cm of a martian soil simulant.…”

Section: Discussionmentioning

confidence: 99%

Mars-like UV Flux and Ionizing Radiation Differently Affect Biomarker Detectability in the Desert Cyanobacterium Chroococcidiopsis as Revealed by the Life Detector Chip Antibody Microarray

et al. 2022

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The effect of a Mars-like UV flux and g-radiation on the detectability of biomarkers in dried cells of Chroococcidiopsis sp. CCMEE 029 was investigated using a fluorescence sandwich microarray immunoassay. The production of anti-Chroococcidiopsis antibodies allowed the immunoidentification of a reduced, though still detectable, signal in dried cells mixed with phyllosilicatic and sulfatic Mars regolith simulants after exposure to 6.8 • 10 5 kJ/m 2 of a Mars-like UV flux. No signal was detected in dried cells that were not mixed with minerals after 1.4 • 10 5 kJ/m 2 . For g-radiation ( 60 Co), no detectable variations of the fluorescence signal occurred in dried cells exposed to 113 kGy compared to non-irradiated dried cells. Our results suggest that immunoassaybased techniques could be used to detect life tracers eventually present in the martian subsurface in freshly excavated materials only if shielded from solar UV. The high structural integrity of biomarkers irradiated with g-radiation that mimics a dose accumulated in 13 Myr at 2 m depth from the martian surface has implications for the potential detectability of similar organic molecules/compounds by future life-detection missions such as the ExoMars Rosalind Franklin rover.

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Section: Discussionmentioning

confidence: 99%

Mars-like UV Flux and Ionizing Radiation Differently Affect Biomarker Detectability in the Desert Cyanobacterium Chroococcidiopsis as Revealed by the Life Detector Chip Antibody Microarray

et al. 2022

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“…Therefore, plant–microorganism–soil interaction is the main driving factor of carbon and nitrogen cycling ( Delgado-Baquerizo et al, 2016 ). Some studies have shown that a certain dose of ionizing radiation will cause changes in plant functional traits and inhibit their growth and development, changes in the content, structure, and properties of organic and inorganic substances in soil, reduction of microbial diversity, and changes in ecosystem structure and function ( Mohammad and Majed, 2019 ; Sergey et al, 2019 ; Cheptsov et al, 2021 ; Videvall et al, 2023 ). However, most of the studies on the ecological effects of ionizing radiation have mostly been carried out under laboratory conditions and usually based on a single species, excluding biological environmental factors and controlling non-biological factors, which makes these results have great limitations ( Geras'kin, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Plant–microorganism–soil interaction under long-term low-dose ionizing radiation

Zeng,

Wen,

Luo

et al. 2024

Front. Microbiol.

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As the environmental nuclear radiation pollution caused by nuclear-contaminated water discharge and other factors intensifies, more plant–microorganism–soil systems will be under long-term low-dose ionizing radiation (LLR). However, the regulatory mechanisms of the plant–microorganism–soil system under LLR are still unclear. In this study, we study a system that has been stably exposed to low-dose ionizing radiation for 10 years and investigate the response of the plant–microorganism–soil system to LLR based on the decay of the absorbed dose rate with distance. The results show that LLR affects the carbon and nitrogen migration process between plant–microorganism–soil through the “symbiotic microbial effect.” The increase in the intensity of ionizing radiation led to a significant increase in the relative abundance of symbiotic fungi, such as Ectomycorrhizal fungi and Rhizobiales, which is accompanied by a significant increase in soil lignin peroxidase (LiP) activity, the C/N ratio, and C%. Meanwhile, enhanced radiation intensity causes adaptive changes in the plant functional traits. This study demonstrates that the “symbiotic microbial effect” of plant–microorganism–soil systems is an important process in terrestrial ecosystems in response to LLR.

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Achievements and Perspectives of Development in Soil Microbiology at Moscow University

STEPANOV,

MANUCHAROVA,

NIKITIN

et al. 2023

Ser-17_2023-4

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The article summarizes the results of recent research by the staff of Soil Biology Department Faculty of Soil Science of Lomonosov Moscow State University in the field of assessing the genetic potential of microbial communities of soils and their application in the development of fundamental soil and environmental technologies. Promising areas of further work related to the use of the microbial potential of soils for the purpose of bioremediation territories from ecotoxicants, the development of technologies for selfpurification of soils based on the stimulation of natural communities of microorganisms, as well as the use of microbial cultures for biodegradation of petroleum products, pesticides and synthetic polymers. Another important direction is related to the development of scientific basis for the indication of biological objects in the environment and space objects. Within the framework of this direction, genomic analysis of uncultivated microorganisms from the Arctic, Antarctic and other extreme habitats is carried out, and the knowledge gained apply as a model of alien life. Another relevant direction for the Department of Soil Biology is the development of agrobiotechnologies based on the management of the natural soil microbiome, the creation of microbial preparationsstimulators of plant growth and development, microbiological ways to increase the proportion of biological nitrogen in plant nutrition, application of microbial plant endosymbionts and bioinsecticides. An equally important aspect is the search of producers of biologically active substances, such as phytohormones, antibiotics, enzymes, probiotics, hydrolytics of natural and artificial polymers. The considered areas of research in the field of soil biology are important for improving land management, environmental protection and the development of environmental technologies.

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Resistance of Enzymes to Ionizing Radiation under Model Conditions of the Martian Regolith

Cited by 3 publications

References 44 publications

Mars-like UV Flux and Ionizing Radiation Differently Affect Biomarker Detectability in the Desert Cyanobacterium Chroococcidiopsis as Revealed by the Life Detector Chip Antibody Microarray

Mars-like UV Flux and Ionizing Radiation Differently Affect Biomarker Detectability in the Desert Cyanobacterium Chroococcidiopsis as Revealed by the Life Detector Chip Antibody Microarray

Plant–microorganism–soil interaction under long-term low-dose ionizing radiation

Achievements and Perspectives of Development in Soil Microbiology at Moscow University

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