Paracoccus beibuensis SL2 is a halo-alkalotolerant bacteria isolated from Lonar Crater, Buldhana, Maharashtra, India and was identified by conventional and advanced techniques. The 16s rRNA sequence was deposited to NCBI GenBank with accession number KY129665. In recent studies the bright orange pigment produced by Paracoccus beibuensis SL2 was extracted and purified. The analysis of purified pigment was done by spectrophotometric method, chemical method, TLC, FT-IR and HPLC to know the chemical nature of pigment. The overall studies showed that bright orange pigment produced by Paracoccus beibuensis SL2 is a carotenoid group pigment which is mainly a xanthophyll pigment showing similar characters with that of Astaxanthin. It has a great commercial value as a natural colorant and therapeutic molecule.
This article includes isolation and screening of carotenoid producing bacteria. Samples were collected from different eco-climatic regions of Maharashtra. From soil, mud, sediment, and water samples total 620 bacterial strains were isolated out of which 105 isolates were pigment producers, many of which exhibited bright orange, orange, yellow orange, lemon yellow and red pigmentation. Out of 105 isolates efficient carotenoid producers were screened after primary, secondary, tertiary, and quaternary screening based on pigmentation, tolerance to pH and NaCl%(w/v), degree of pigmentation, absorption maxima and free radicle scavenging activity respectively. The selection of potential carotenoid producer was done from finally screened isolatesSL2, SL4 * , SL4-1, SL10, SL2y, DKMIII, DABW1, DGM, SSD2, SWT3 and SS1 based on free radicle scavenging activity by DPPH (1,1 Diphenyl-2 Picryl Hydrazil) method. Isolate SL2 showed bright orange pigmentation, tolerance to pH (6-12), NaCl (1-6%), λmax at 473 nm and maximum 53% free radicle scavenging activity, so it was selected for further studies as a potential carotenoid pigment producer.
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