Isolation and Screening of Carotenoid Producing Bacteria

Abstract

This article includes isolation and screening of carotenoid producing bacteria. Samples were collected from different eco-climatic regions of Maharashtra. From soil, mud, sediment, and water samples total 620 bacterial strains were isolated out of which 105 isolates were pigment producers, many of which exhibited bright orange, orange, yellow orange, lemon yellow and red pigmentation. Out of 105 isolates efficient carotenoid producers were screened after primary, secondary, tertiary, and quaternary screening based on pigmentation, tolerance to pH and NaCl%(w/v), degree of pigmentation, absorption maxima and free radicle scavenging activity respectively. The selection of potential carotenoid producer was done from finally screened isolatesSL2, SL4 * , SL4-1, SL10, SL2y, DKMIII, DABW1, DGM, SSD2, SWT3 and SS1 based on free radicle scavenging activity by DPPH (1,1 Diphenyl-2 Picryl Hydrazil) method. Isolate SL2 showed bright orange pigmentation, tolerance to pH (6-12), NaCl (1-6%), λmax at 473 nm and maximum 53% free radicle scavenging activity, so it was selected for further studies as a potential carotenoid pigment producer.