Solid tumor is generally observed in tissues of epithelial or endothelial cells of lung, breast, prostate, pancreases, colorectal, stomach, and bladder, where several genes transcription is regulated by the microRNAs (miRNAs). Argonaute (AGO) protein is a family of protein which assists in miRNAs to bind with mRNAs of the target genes. Hence, study of the binding mechanism between AGO protein and miRNAs, and also with miRNAs-mRNAs duplex is crucial for understanding the RNA silencing mechanism. In the current work, 64 genes and 23 miRNAs have been selected from literatures, whose deregulation is well established in seven types of solid cancer like lung, breast, prostate, pancreases, colorectal, stomach, and bladder cancer. In silico study reveals, miRNAs namely, miR-106a, miR-21, and miR-29b-2 have a strong binding affinity towards PTEN, TGFBR2, and VEGFA genes, respectively, suggested as important factors in RNA silencing mechanism. Furthermore, interaction between AGO protein (PDB ID-3F73, chain A) with selected miRNAs and with miRNAs-mRNAs duplex were studied computationally to understand their binding at molecular level. The residual interaction and hydrogen bonding are inspected in Discovery Studio 3.5 suites. The current investigation throws light on understanding miRNAs based gene silencing mechanism in solid cancer.

The present experiment was carried ut to assess the comparative e cacy of ivermectin and fenbendazole individually for anthelmintic therapy for the hookworm infested dogs. Dogs presented to the Department of Veterinary Clinical Medicine or Veterinary Clinical Complex, Bhubaneswar were randomly screened for Ancylostoma caninum infection and the positive dogs were selected for the therapeutic trial Faecal samples were collected randomly from presented dogs immediately after defaecation or from the rectum directly using a faecal scoop. The collected sample was examined by oatation technique to detect the positive cases of Ancylostoma caninum infection. The dogs with normal clinical parameters and no eggs or ova in the faeces were included in in group 1 (n=12). Dogs with faecal sample positive for Ancylostoma caninum ova were recruited for the comparative study (n=24) which were grouped into two groups consisting of 12 dogs in each (group 2 and 3). Group 2 dogs (n=12) were treated with ivermectin at 200 µg/kg body weight once orally repeated after 15 days with proper supportive therapy each time. Group 3 (n=12) were treated with fenbendazole at 50 mg/kg body weight once orally repeated after 15 days with proper supportive therapy each time.Haematological examinations and serum biochemical tests were carried out in all groups each time on day 0, 15 and 30 of the experiment. The therapeutic e cacy of both the drugs was calculated on the basis of number of animals found free of Ancylostoma infection as determined by faecal sample examination and reduction in EPG count of the faeces of the group following the treatment. The reduction in eggs per gram (EPG) count on day 15 and day 30 was more signi cant in group 2 than group 3. The mean EPG count in fenbendazole-treated dogs reduced signi cantly to 24.17 + 11.44 on day 15 from day 0 level of 1650.00 + 247.25. The value again reduced from day 0 to become nil on day 30 .The 15th day after treatment, mean ± SE values of protein, albumin and globulin were changed to 5.63 ± 0.12, 2.64 ± 0.12 and 2.99 ± 0.15 g/dl, respectively. The 30th day after treatment the values were 6.23 ± 0.14, 3.20 ± 0.18 and 3.03 ± 0.21 g/dl, respectively. The total protein and albumin values were signi cantly changed by 15th day and 30th day at 1% level of signi cance as compared to that of day 0 in group 2 and 3 respectively.Following treatment with ivermectin, the 15 th day haematological values increased signi cantly at 1% level (P<0.01) of signi cance. There was signi cant increase in the values at 1% level on the 30 th day compared to day 0. The mean values were non-signi cantly comparable to the healthy control group except PCV and TEC on the 30 th day.

A study was conducted to evaluate the performance of Vezaguda, an indigenous chicken population of Odisha, up to 8 weeks of age in floor rearing system. Unsexed day-old chicks (100) were selected for this study. Mortality and feed intake were recorded daily while body weight was recorded weekly. Body conformation traits such as shank length, shank circumference, shank width, thigh length, chest girth, keel length, body length, height, back length, wing length, folded wing length, wing span, neck length, head length, skull length, head width, beak length and breast angle were measured at 6th and 8th weeks of age. The 8th week body weight of male and female chicks were 501.58±21.64 g and 423.35±13.44 g, respectively. The cumulative feed conversion ratio (FCR), energy efficiency ratio (EER), and protein efficiency ratio (PER) at 8th week were 4.00±0.06, 8.73±0.12, and 1.25±0.02, respectively. An index of productivity, production efficiency factor (PEF) was calculated taking into account final weight, liveability %, age of the bird and FCR. The PEF at 8th week was 16.38±0.46. At 6th week of age, all recorded conformation traits were higher in male chicks than that of female chicks. At 8th week of age, chest girth, skull length and beak length were significantly higher in male chicks than that of female chicks.

The present study was established to establish a protocol for in vitro micropropagation of Costus igneus and to investigate anti diabetic activity through in silco analysis. Direct Shoot regeneration occurred using the nodal and rhizome part of explants in MS medium supplemented with BAP (1 mg/l) + NAA (0.5 mg/l) show 77% shoots induction with 6.1cm in length. Among them the plant growth regulators (PGRs) BAP (1mg/l) and NAA (1mg/l) supplemented with MS medium had the maximum rooting about 78% and 11.5±0.04 no of roots. The acclimatization of rooted plants in ex vitro conditions was carried out with the plants bearing well-developed roots transferred to small pots containing soil mixtures (organic soil mixed with garden soil 1:1). They were maintained at about 70% relative humidity in the greenhouse with 75% shading. A survival rate 80 % was achieved after 6 weeks. The integration of computational and experimental strategies was great value in the identification and development of novel promising compounds. Broadly used in modern drug design, molecular docking methods explore the ligand conformations adopted within the binding sites of macromolecular targets. Phytocompounds present in the plant was done to find the appropeate ligand against INS and TNF α which were important genes associated with diabetes. From the docking results the best ligand was found to be strictinin with-12.17 binding energy when interacted with INS and-10.79 binding energy when interacted with TNF α.