In Silico Analysis of Micro Propagated Endangered Species Costus Igneus

Abstract

The present study was established to establish a protocol for in vitro micropropagation of Costus igneus and to investigate anti diabetic activity through in silco analysis. Direct Shoot regeneration occurred using the nodal and rhizome part of explants in MS medium supplemented with BAP (1 mg/l) + NAA (0.5 mg/l) show 77% shoots induction with 6.1cm in length. Among them the plant growth regulators (PGRs) BAP (1mg/l) and NAA (1mg/l) supplemented with MS medium had the maximum rooting about 78% and 11.5±0.04 no of roots. The acclimatization of rooted plants in ex vitro conditions was carried out with the plants bearing well-developed roots transferred to small pots containing soil mixtures (organic soil mixed with garden soil 1:1). They were maintained at about 70% relative humidity in the greenhouse with 75% shading. A survival rate 80 % was achieved after 6 weeks. The integration of computational and experimental strategies was great value in the identification and development of novel promising compounds. Broadly used in modern drug design, molecular docking methods explore the ligand conformations adopted within the binding sites of macromolecular targets. Phytocompounds present in the plant was done to find the appropeate ligand against INS and TNF α which were important genes associated with diabetes. From the docking results the best ligand was found to be strictinin with-12.17 binding energy when interacted with INS and-10.79 binding energy when interacted with TNF α.