A topical antimicrobial, silver oxynitrate (AgNO), has recently become available that exploits the antimicrobial activity of ionic silver but has enhanced activity because highly oxidised silver atoms are stabilised with oxygen in a unique chemical formulation. The objective of this study was to use a multifaceted approach to characterise the spectrum of antimicrobial and antibiofilm activity of a wound dressing coated with AgNO at a concentration of 0.4 mg Ag/cm. Physiochemical properties that influence efficacy were also evaluated, and AgNO was found to release a high level of Ag ions, including Ag and Ag, without influencing the pH of the medium. Time-kill analysis demonstrated that a panel of multidrug-resistant pathogens isolated from wound specimens remained susceptible to AgNO over a period of 7 days, even with repeated inoculations of 1 × 10 CFU/mL to the dressing. Furthermore, established 72-h-old biofilms of Pseudomonas aeruginosa, Staphylococcus aureus and two carbapenem-resistant Gram-negative bacteria (bla-positive Klebsiella pneumoniae and bla-positive P. aeruginosa) were disrupted and eradicated by AgNO in vitro. AgNO is a proprietary compound that exploits novel Ag chemistry and can be considered a new class of topical antimicrobial agent. Biocompatibility testing has concluded AgNO to be non-toxic for cytotoxicity, acute systemic toxicity, irritation and sensitisation.
Although identifying specific microbial taxa associated with obesity and type 2 diabetes remains difficult, certain bacteria may play an important role in initiating metabolic inflammation during disease development. Here, we used a mouse model distinguishable by the presence or absence of a commensal
Escherichia coli
strain in combination with a high-fat diet challenge to investigate the impact of
E. coli
on host metabolic outcomes.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.