Background: Sirtuin-3 (Sirt3) has been documented to protect against mitochondrial dysfunction and apoptosis. Honokiol (HKL) is a Sirt3 pharmacological activator with reported neuroprotective effects in multiple neurological disorders. The present study aimed to explore the neuroprotective effects of HKL and the role of Sirt3 following intracerebral hemorrhage (ICH). Methods: An in vivo ICH model in rats was established by injecting autologous blood into the right basal ganglia. PC12 cells were stimulated with hemin. For the in vivo investigation, the modified Neurological Severity Scores and the Morris water maze test were performed to assess neurological deficits. Hematoxylin–Eosin and Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling staining were employed to evaluate the histopathology and apoptosis. Immunohistochemical staining was used to investigate the expression of Sirt3. Adenosine triphosphate (ATP) levels were quantified to assess mitochondrial dysfunction. Cell counting kit-8, lactate dehydrogenase assay, and flow cytometry were used to analyze cell vitality and apoptosis in vitro. Immunofluorescence staining was performed to observe mitochondrial morphology and dynamin-related protein 1 (Drp1) localization to mitochondria. Western blot was applied to quantify the expression of Sirt3, Bax, Bcl-2, cleaved-caspase-3, Drp1, phosphorylation of Drp1 at serine-616, and phosphorylation of Drp1 at serine-637 in vivo and in vitro. Results: HKL treatment alleviated neurological deficits, attenuated the histopathological damage and cell apoptosis, and restored the decreased ATP levels in ICH rats. HKL improved cell survival rate, reduced cell apoptosis, and inhibited mitochondrial fission in PC12 cells. Moreover, both in vivo and in vitro models showed increased phosphorylation of Drp1 at Ser616, and reduced phosphorylation of Drp1 at Ser637. Meanwhile, immunofluorescence co-localization analysis revealed that hemin increased the overlap of Drp1 and mitochondria in PC12 cells. The phosphorylation and mitochondrial translocation of Drp1 were effectively reversed by HKL treatment. Importantly, the selective Sirt3 inhibitor 3-(1H-1,2,3-triazol-4-yl) pyridine suppressed these effects. Conclusion: Our findings demonstrated that HKL ameliorated ICH-induced apoptosis and mitochondrial fission by Sirt3, suggesting that HKL has immense prospects for the treatment of ICH.
In order to make the resistance underwater as small as possible, the shape of cavitator has been studied, and supercavitation resistance and drag reduction which related to the foreign experimental results is discussed. In this paper, first the super-cavity underwater body shape is optimized. we analyzed the super-cavity flow model and has gain cavity's radius function in the optimization, then use the path of least resistance, which result in Mathematical modeling with the largest cavity volume by the double goals of optimizing, and the constraints are given.
The steady bubble shape as one of the basic research, plays a decisive role in the cavitation’s study, To study it clearly will be not only basis for the study of non-steady cavitation, but also provide the necessary basis for the design of the control for cavitation bubble. In this paper, cavity shape produced by submerged body is studied, and related to the use of foreign experimental results, super-cavitation resistance, drag reduction is discussed.
To improve the situation of stress concentration in the combustion chamber component, optimized the design of combustion chamber shell structures is required. In this paper, a new boundary element integrated optimization method is used. There are a symmetry axis structure and effective constrained optimization model for sensitivity analysis. The technology and the general shape optimization algorithm are combined. The components' shape of the combustion chamber's shell under a plane stress is optimized, and finally, the satisfactory results are obtained and the maximum tangential bore stress is reduced.
The Genetic Algorithms In engineering structure optimization design includes Truss Structure optimization, Shape and topology optimization, Composite materials optimization, layout optimization, Multi-Objective Optimization. This paper combined with engineering background , Selecting the Pressing gear of CNC crushing Machine Steady as starting point for Optimization modeling .Analyzing the simple conditions theoretical physical model of CNC Crushing Machine Steady, Reasonably selected design variables ,using Conventional Methods and genetic algorithms to optimize the Steady ,obtainning every iterative step relevant data under the two methods, and analyzing the results ,analysis the accuracy of the optimize results through the stress and displacement map .
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