Wound healing is a multifaceted biological process involving the replacement of damaged tissues and cellular structures, restoring the skin barrier's function, and maintaining internal homeostasis. Over the past two decades, numerous approaches are undertaken to improve the quality and healing rate of complex acute and chronic wounds, including synthetic and natural polymeric scaffolds, skin grafts, and supramolecular hydrogels. In this context, this review assesses the advantages and drawbacks of various types of supramolecular hydrogels including both polymeric and peptide‐based hydrogels for wound healing applications. The molecular design features of natural and synthetic polymers are examined, as well as therapeutic‐based and drug‐free peptide hydrogels, and the strategies for each system are analyzed to integrate key elements such as biocompatibility, bioactivity, stimuli‐responsiveness, site specificity, biodegradability, and clearance.
The
separation and purification of therapeutic proteins from their
biological resources pose a great limitation for industrial manufacturing
of biologics in an efficient and cost-effective manner. We report
here a supramolecular polymeric system that can undergo multiple reversible
processes for efficient capture, precipitation, and recovery of monoclonal
antibodies (mAbs). These supramolecular polymers, namely immunofibers
(IFs), are formed by coassembly of a mAb-binding peptide amphiphile
with a rationally designed filler molecule of varying stoichiometric
ratios. Under the optimized conditions, IFs can specifically capture
mAbs with a precipitation yield greater than 99%, leading to an overall
mAb recovery yield of 94%. We also demonstrated the feasibility of
capturing and recovering two mAbs from clarified cell culture harvest.
These results showcase the promising potential of peptide-based supramolecular
polymers as reversible affinity precipitants for mAb purification.
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