Selective Capture and Recovery of Monoclonal Antibodies by Self-Assembling Supramolecular Polymers of High Affinity for Protein Binding

Abstract

The separation and purification of therapeutic proteins from their biological resources pose a great limitation for industrial manufacturing of biologics in an efficient and cost-effective manner. We report here a supramolecular polymeric system that can undergo multiple reversible processes for efficient capture, precipitation, and recovery of monoclonal antibodies (mAbs). These supramolecular polymers, namely immunofibers (IFs), are formed by coassembly of a mAb-binding peptide amphiphile with a rationally designed filler molecule of varying stoichiometric ratios. Under the optimized conditions, IFs can specifically capture mAbs with a precipitation yield greater than 99%, leading to an overall mAb recovery yield of 94%. We also demonstrated the feasibility of capturing and recovering two mAbs from clarified cell culture harvest. These results showcase the promising potential of peptide-based supramolecular polymers as reversible affinity precipitants for mAb purification.

Recent Developments of Liquid Chromatography Stationary Phases for Compound Separation: From Proteins to Small Organic Compounds

“…For instance, the high selectivity of human IgG over human serum albumin was achieved using alginate dialdehyde layers on a nylon membrane, which significantly reduced nonspecific adsorption [ 62 ]. Moreover, peptides that selectively bind monoclonal antibodies, such as the immunofibers, could also be introduced on the stationary phase surface [ 63 ].…”

Molecularly Imprinted Affinity Membrane: A Review

“… 54 Another example is the introduction of peptides on membrane surfaces to obtain high selectivity toward monoclonal antibodies, such as immunofibers. 55 …”

“…For instance, when nonspecific adsorption is reduced significantly, a nylon membrane with alginate dialdehyde layers can achieve high selectivity of human IgG over human serum albumin . Another example is the introduction of peptides on membrane surfaces to obtain high selectivity toward monoclonal antibodies, such as immunofibers …”

“…54 Another example is the introduction of peptides on membrane surfaces to obtain high selectivity toward monoclonal antibodies, such as immunofibers. 55 Affinity membranes have also been investigated regarding virus and bacteria capture. A sulfated cellulose membrane was employed to bind the influenza virus, resulting in a 75% viralparticle capture while reducing protein and DNA contamination to <25%.…”

Intrinsic Fluorescence in Peptide Amphiphile Micelles with Protein-Inspired Phosphate Sensing

“…One quintessential biomimetic material is peptide amphiphile micelles (PAMs). − Peptide amphiphiles (PAs) consist of a peptide headgroup conjugated to a hydrophobic tail that spontaneously self-assembles into micelles in water to display the peptide to the aqueous environment. PAMs fuse strategic material design and fundamental study with a diverse and creative array of applications including targeted drug delivery, protein purification, nutrient reclamation, nerve regeneration, and tissue scaffolding for spinal cord recovery . This wide breadth of functionality is directly linked to tunable material design advantages.…”