Actinide Structural Studies. Part 7.' The Crystal and Molecular Structures of (2,2'-Bipyridyl)dinitratodioxo-~raniurn(~1) and -neptunium(vi), and Diacetato-(2,2'-bi pyridyl )d ioxo-u ran i u m( VI) and -neptunium (vi) t

The aim of this study was to demonstrate proof-of-concept feasibility for the use of human neural stem cells (NSCs) for highthroughput screening (HTS) applications. For this study, an adherent human induced pluripotent stem (iPS) cell-derived long-term, self-renewing, neuroepithelial-like stem (lt-NES) cell line was selected as a representative NSC. Here, we describe the automated large-scale serum-free culture ("scale-up") of human lt-NES cells on the CompacT SelecT cell culture robotic platform, followed by their subsequent automated "scale-out" into a microwell plate format. We also report a medium-throughput screen of 1000 compounds to identify modulators of neural stem cell proliferation and/or survival. The screen was performed on two independent occasions using a cell viability assay with end-point reading resulting in the identification of 24 potential hit compounds, 5 of which were found to increase the proliferation and/or survival of human lt-NES on both occasions. Follow-up studies confirmed a dosedependent effect of one of the hit compounds, which was a Cdk-2 modulator. This approach could be further developed as part of a strategy to screen compounds to either improve the procedures for the in vitro expansion of neural stem cells or to potentially modulate endogenous neural stem cell behavior in the diseased nervous system.

Pb(S2COCH3)2] , M r = 421.5, monoclinic, C2, a = 12.285 (5), b --4.2596 (7), c = 10-060 (3) A, r= 108.04 (3) °, V= 500 (2) A 3, D x = 2.800 Mg m -3 0108-2701/88/020250-04503.00for Z = 2, ;t(Mo K~) = 0.7107 A, p = 17.693 mm-', F(000) = 384, T= 295 (2) K, R = 0.058 for 455 observed reflections. The Pb atom in Pb(S2COCH3) 2 is

Bis(1,3-diphenyl-l,3-propanedionato)oxo-(pyridine)uranium(VI) (1), [UO2(CasHllO2)2(CsHsN)], Mr= 795.6, monoclinic, P2Jn, a= 10.158 (2), b= 21.937 (8), c= 13.888 (4)A, t= 103.49 (1) ° , U = 3009 (2)/k 3, Z = 4, D x = 1.76 g cm -3, 2(Mo Ka) = 0.71069 A, /t = 51.48 cm -1, F(000) = 1536, T= 289 K, R = 0.036 for 3269 unique observed reflections. Bis[ 1-(tert-butoxy)-1,3-butanedionato]dioxo-(pyridine)uranium(IV) (2), [UO2(CaHI303)2(CsHsN)], M r= 663.5, orthorhombic, Cmc2~, a = 15.939 (9), b = 19.532 (8), c= 9.777 (6) A, U= 3044 (1) A 3, Z = 4, D x = 1.45 gcm -a, 2(Mo Kct) = 0.71069/~, /t = 50.81 cm -1, F(000) = 1280, T= 289 K, R = 0.048 for 1178 unique observed reflections. Dioxobis(2,4-pentanedionato)(pyridine)neptunium(VI) (3), [NpO2(CsH 7-O2)2(CsHsN)], Mr=546.4, orthorhombic, Fdd2, a = 29.617 (5), b= 11.406 (2), c= 10.595 (4)A, U= 3579 (2) A 3, Z=8, D x=2.03gcm -3, 2(MoKa)= Acta Cryst. Abstract.