Background: There are high mortality and morbidity rates due to poisonous snakebites globally with sub-Saharan Africa having some of the highest cases. However, traditional medicine practitioners (TMP) have been treating snakebites in Uganda for long despite the fact that few studies have been conducted to document such vital and rich indigenous traditional knowledge before it is lost. This study aimed to document the medicinal plant species used by experienced TMP in treating snakebite envenomation in selected post-conflict parts of Uganda. An ethnopharmacological survey was conducted in Kitgum, Serere, Kaberamaido and Kaabong districts in Uganda. Twenty-seven TMP with expertise in treating snakebites were purposively identified using the snowball technique and interviewed using semi-structured questionnaires. Data were analysed using simple descriptive statistics. Results: Sixty plant species from 28 families were documented with high consensus among the isolated indigenous Ik tribe of Kaabong district. Most of the plant species used were from the Asteraceae and Fabaceae families with eight species each. The genus Echinops was the most well-represented with three species. The most commonly used plant species were of citation were Steganotaenia araliaceae (16), Microglossa pyrifolia (Lam.), Gladiolus dalenii Van Geel (13), Aframomum mildbraedii Loes. (11), Jasminum schimperi Vatke and Cyathula uncinulata (Schrad) Schinz (10) and Crinum macowanii Baker and Cyphostemma cyphopetalum (Fresen.) Desc. ex Wild & R.B. Drumm (10). S. araliaceae which was mentioned by all the TMP in the Ik community was used for first aid. Most of the plant species were harvested from the wild (68.75%) and were herbs (65.0%) followed by trees (23.3%). The most commonly used plant parts were roots (42.6%) and leaves (25.0%). Thirteen different methods of preparation and administration were used. Most of the medicines were administered orally (61.2%) and topically (37.6%). The commonest methods of oral application were cold water infusions (32.5%) and decoctions (21.7%). Conclusions: TMP widely use several medicinal plant species for treating snakebite envenomation in the selected post-conflict regions of Uganda
Plastics have become an essential part of modern life today. The global production of plastics has gone up to 299 million tonnes in 2013, which has increased enormously in the present years. The utilization of plastics and its final disposal pose tremendous negative significant impacts on the environment. The present study aimed to investigate the thermal and catalytic pyrolysis for the production of fuel oil from the polyethene plastic wastes. The samples collection for both plastic wastes and clay catalyst, sample preparation and pyrolysis experiment for oil production was done in Laroo Division, Gulu Municipality, Northern Uganda Region, Uganda. Catalysts used in the experiment were acid-activated clay mineral and aluminium chlorides on activated carbon. The clay mineral was activated by refluxing it with 6M Sulphuric acid for 3 hours. The experiment was conducted in three different phases: The first phase of the experiment was done without a catalyst (purely thermal pyrolysis). The second phase involves the use of acid-activated clay mineral. The third phase was done using aluminium chlorides on activated carbon. Both phases were done at different heating rates. In purely thermal pyrolysis, 88 mL of oil was obtained at a maximum temperature of 39ºC and heating rates of 12.55ºC /minute and reaction time of 4 hours. Acid activated clay mineral yielded 100 mL of oil with the heating rates of 12.55ºC/minute and reaction time of 3 hours 30 minutes. While aluminium chlorides on activated carbon produced 105 mL of oil at a maximum temperature of 400ºC and heating rates of 15.5ºC /minute and reaction time of 3 hours 10 minutes. From the experimental results, catalytic pyrolysis is more efficient than purely thermal pyrolysis and homogenous catalysis (aluminium chlorides) shows a better result than solid acid catalyst (activated clay minerals) hence saving the energy needed for pyrolysis and making the process more economically feasible.
There are high mortality and morbidity rates from poisonous snakebites globally. Many medicinal plants are locally used for snakebite treatment in Uganda. This study aimed to determine the in vitro anti-venom activities of aqueous extract and oils of Toona ciliata against Naja melanoleuca venom. A mixture of venom and extract was administered intramuscularly in rats. Anticoagulant, antiphospholipase A2 (PLA2) inhibition assay, and gel electrophoresis for anti-venom activities of oils were done. The chemical constituents of the oils of ciliata were identified using Gas chromatography-tandem mass spectroscopy (GC-MS/MS). The LD50 of the venom was 0.168 ± 0.21 µg/g. The venom and aqueous extract mixture (1.25 µg/g and 3.5 mg/g) did not cause any rat mortality, while the control with venom only (1.25 µg/g) caused death in 1 h. The aqueous extract of T. ciliata inhibited the anticoagulation activity of N. melanoleuca venom from 18.58 min. to 4.83 min and reduced the hemolytic halo diameter from 24 to 22 mm. SDS-PAGE gel electrophoresis showed that oils completely cleared venom proteins. GC-MS/MS analysis showed that the oils had sesquiterpene hydrocarbons (60%) in the volatile oil (VO) and oxygenated sesquiterpenes (48.89%) in the non-volatile oils (NVO). Some major compounds reported for the first time in T. ciliata NVOs were: Rutamarin (52.55%), β-Himachalol (9.53%), Girinimbine (6.68%) and Oprea1 (6.24%). Most compounds in the VO were reported for the first time in T. ciliata, including the major ones Santalene (8.55%) and Himachal-7-ol (6.69%). The result showed that aqueous extract and oils of T. ciliata have anti-venom/procoagulant activities and completely neutralized the venom. We recommend a study on isolation and testing the pure compounds against the same venom.
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