A rapid capillary gas chromatographic method was developed for determination of supplemental cc-tocopherol acetate (containing and verified at 50–5000 mg/kg feed) in various kinds of feed premixes. The method is based on simple n-hexane extraction of feed samples pretreated with a solution of acetic acid in ether for matrix breakdown followed by column cleanup on deactivated alumina to eliminate background interference. Fused silica chromatography is completed in 3 min. The withinday precision expressed as the relative standard deviation for recovery examples was 3.4,2.9, and 4.0% for high, medium, and low concentrated feed premixes, respectively; extraction recovery was 99–110%. The storage stabilities of standards and feed premix preparations were found to be excellent up to 3 months and up to 60° C.
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