Polyhydroxyalkanoates (PHA) are biodegradable polyesters accumulated intracellularly as energy resources by bacterial species. In this study, fermentation process for production of PHA is carried out using sesame oil as carbon source. We studied recovery of poly(3-hydroxybutyric acid) (PHB) from Staphylococcus epidermidis by sodium hypochlorite digestion method. Recovered PHB sample was estimated by UV spectrophotometer. PHB from S. epidermidis was characterized and by these findings, we examined purified PHB by differential scanning calorimeter (DSC), a thermo gravimetric analyzer (TGA), thin layer chromatography (TLC) and infrared spectroscopy (IR). The results of our analysis of PHB while comparing with commercial source suggest that in DSC melting temperature of PHB was 173.36°C, TGA thermo grams of PHB sample was at 296.91°C, on TLC plate; Rf value was calculated as 0.71 and finally IR spectrum of the compounds showed characteristics bands for the groups CH, C=O and CO , indicating the presence of PHB in the production medium.
Amylases are the enzymes that hydrolyse starch or glycogen and produce, polymers of glucose subunits. α-amylases are one of the important and enzyme in many sectors such asclinical, medicinal and analytical chemistry. Besides their use in starch saccaharification they also find applications in food, baking, brewing, detergent, textile, paper, and distilling industry. Amylase can be derived from several sources, such as plants, microorganisms. Amylase can be produce from economically available agricultural starchy substrate using bacteria and fungi. Different agricultural starchy substrates such as soluble starch hordium, pearl millet, rice, corn, gram and wheat starch, banana peel, potato peel coconut oil cake, sesame oil cake, groundnut oil cake, palm kernel cake and olive oil cake were used for production of α-amylase using different microorganisms.
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