BackgroundTrehalase, an enzyme that hydrolyzes trehalose to yield two glucose molecules, plays a pivotal role in various physiological processes. In recent years, trehalase proteins have been purified from several insect species and are divided into soluble (Tre-1) and membrane-bound (Tre-2) trehalases. However, no functions of the two trehalases in chitin biosynthesis in insects have yet been reported.Principal FindingsThe membrane-bound trehalase of Spodoptera exigua (SeTre-2) was characterized in our laboratory previously. In this study, we cloned the soluble trehalase gene (SeTre-1) and investigated the tissue distribution and developmental expression pattern of the two trehalase genes. SeTre-1 was expressed highly in cuticle and Malpighian tubules, while SeTre-2 was expressed in tracheae and fat body. In the midgut, the two trehalase genes were expressed in different locations. Additionally, the expression profiles of both trehalase mRNAs and their enzyme activities suggest that they may play different roles in chitin biosynthesis. The RNA interference (RNAi) of either SeTre-1 or SeTre-2 was gene-specific and effective, with efficiency rates up to 83% at 72 h post injection. After RNAi of SeTre-1 and SeTre-2, significant higher mortality rates were observed during the larva-pupa stage and pupa-adult stage, and the lethal phenotypes were classified and analyzed. Additionally, the change trends of concentration of trehalose and glucose appeared reciprocally in RNAi-mutants. Moreover, knockdown of SeTre-1 gene largely inhibited the expression of chitin synthase gene A (CHSA) and reduced the chitin content in the cuticle to two-thirds relative to the control insects. The chitin synthase gene B (CHSB) expression, however, was inhibited more by the injection of dsRNA for SeTre-2, and the chitin content in the midgut decreased by about 25%.Conclusions SeTre-1 plays a major role in CHSA expression and chitin synthesis in the cuticle, and SeTre-2 has an important role in CHSB expression and chitin synthesis in the midgut.

Intermittent food shortages are commonly encountered in the wild. During winter or starvation stress, mammals often choose to hibernate while insects—in the form of eggs, mature larvae, pupae, or adults opt to enter diapause. In response to food shortages, insects may try to find sufficient food to maintain normal growth and metabolism through distribution of populations or even migration. In the face of hunger or starvation, insect responses can include changes in behavior and/or maintenance of a low metabolic rate through physiological adaptations or regulation. For instance, in order to maintain homeostasis of the blood sugar, trehalose under starvation stress, other sugars can be transformed to sustain basic energy metabolism. Furthermore, as the severity of starvation increases, lipids (especially triglycerides) are broken down to improve hunger resistance. Starvation stress simultaneously initiates a series of neural signals and hormone regulation processes in insects. These processes involve neurons or neuropeptides, immunity-related genes, levels of autophagy, heat shock proteins and juvenile hormone levels which maintain lower levels of physiological metabolic activity. This work focuses on hunger stress in insects and reviews its effects on behavior, energy reserve utilization, and physiological regulation. In summary, we highlight the diversity in adaptive strategies of insects to hunger stress and provides potential ideas to improve hunger resistance and cold storage development of natural enemy insects. This gist of literature on insects also broadens our understanding of the factors that dictate phenotypic plasticity in adjusting development and life histories around nutritionally optimal environmental conditions.

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The Paleogene Lulehe Formation marks the onset of deposition in the Qaidam basin and preserves evidence of the initial topographic growth of northern Tibet. However, limited outcrops impede understanding of the sedimentary features of the Lulehe Formation as well as the tectonic relationship between the basin and surrounding topography. To fill this gap, we investigated core samples along the basin margin and conducted flexural modeling to estimate the topographic load of the Qilian Shan and Eastern Kunlun Shan during the deposition of the Lulehe Formation. Core samples reveal that the Lulehe Formation mainly consists of distal fluvial to marginal lacustrine deposits and proximal fluvial deposits along the southern margin of the basin while characterized by proximal alluvial fan deposits along the northern margin of the basin. Together with evidence for faulting shown on the seismic profiles, we infer that simultaneous deformation within the Qilian Shan and Altyn Tagh Shan during the Paleogene resulted in accumulation of coarse detrital deposits in the northwestern and northeastern Qaidam basin. The simultaneous deformation within the Altyn Tagh Shan and Qilian Shan since the Paleogene supports the idea that deformation in these two regions is kinematically linked. One-and two-load beam flexural modeling indicates that the topographic load generated by both the Eastern Kunlun Shan and the Qilian Shan is responsible for the subsidence of the Qaidam basin during deposition of the Lulehe Formation. Our results highlight the initial relative high topography in the northern Tibetan plateau during the early Cenozoic.

Two debated age models, with a basal age of ~50 Ma versus ~30 Ma, are proposed for the depositional age of Cenozoic strata within the Qaidam basin result in a diverse understanding of the initial pattern of deformation in the northern Tibetan Plateau. To evaluate these age models, we integrated isopach maps within the basin with published thermochronology data from surrounding ranges to balance the sediments preserved in the basin with materials eroded in the drainage area. When following the traditional ~50 Ma age model, the total volume of material eroded from the surrounding source area is 4.4 ± 0.3 × 105 km3. Using instead the ~30 Ma age model for the basal Lulehe Formation and related revisions to the basin chronology, the volume of eroded material is calculated at 3.5 ± 0.2 × 105 km3, which provides a better match to the calculated total volume of solid grains that are preserved in the basin (2.8 ± 0.1 × 105 km3). However, growth strata revealed in seismic profiles along the Southern Qaidam Thrust suggest reverse‐faulting began during the deposition of Oligocene‐Miocene strata. Following the ~50 Ma age model, the onset time of faulting along the Southern Qaidam Thrust is ~35.5 Ma, consistent with previous thermochronology results. If both age models are correct, then this requires a significant time‐transgressive nature to basin fill that allows for older ages of deposition in the southern part of the basin. This study highlights the need for further effort to determine the depositional age of the strata in the southern and western parts of the Qaidam basin.