Background
The tumor immune microenvironment plays a crucial role in the efficacy of various therapeutics. However, their correlation is not yet completely understood in Clear cell renal cell carcinoma (ccRCC). This study aimed to investigate the potential of TREM-1 as a potential novel biomarker for ccRCC.
Methods
We constructed a ccRCC immune prognostic signature. The clinical characteristics, the status of the tumor microenvironment, and immune infiltration were analyzed through the ESTIMATE and CIBERSORT algorithms for the hub gene, while the Gene Set Enrichment Analysis and PPI analysis were performed to predict the function of the hub gene. Immunohistochemical staining was used to detect the expression of TREM-1 in renal clear cell carcinoma tissues.
Results
The CIBERSORT and ESTIMATE algorithms revealed that TREM-1 was correlated with the infiltration of 12 types of immune cells. Therefore, it was determined that TREM-1 was involved in numerous classical pathways in the immune response via GSEA analysis. In Immunohistochemical staining, we found that the expression of TREM-1 was significantly upregulated with increasing tumor grade in renal clear cell carcinoma, and elevated TREM-1 expression was associated with poor prognosis.
Conclusions
The results suggest that TREM-1 may act as an implicit novel prognostic biomarker in ccRCC that could be utilized to facilitate immunotherapeutic strategy.
Background: This study aims to find immune-related hub gene as potential novel biomarker for KIRC.
Methods: We constructed a KIRC immune prognostic signature. The receiver operating characteristic (ROC) curves, survival curves, univariate and multivariate Cox regression analysis were utilized to validate the signature. Immunohistochemical staining was used to detect the expression of TRME1 in renal clear cell carcinoma tissues. The clinical characteristics, the status of the tumor microenvironment, and immune infiltration were analyzed through the ESTIMATE and CIBERSORT algorithms for the hub gene, while the Gene Set Enrichment Analysis and PPI analysis were performed to predict the function of the hub gene.
Results: In Immunohistochemical staining, we found that the expression of TREM1 was significantly upregulated with increasing tumor grade in renal clear cell carcinoma, and elevated TREM1 expression was associated with poor prognosis.The CIBERSORT and ESTIMATE algorithms revealed that TREM1 was correlated with the infiltration of 12 types of immune cells. Therefore, it was determined that TREM1 was involved in numerous classical pathways in the immune response via GSEA analysis.
Conclusions: The results suggest that TREM1 may act as an implicit novel prognostic biomarker in KIRC that could be utilized to facilitate immunotherapeutic strategy.
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