Atopic dermatitis is an inflammatory skin disease that may be chronic and recurrent. Staphylococcus aureus infection is the most common cause of the disease due to toxin production by this bacterium. Antibiotic use is increasing over the years, and their indiscriminate use is linked to bacterial resistance. Therefore, the development of effective alternatives to the practice of antibiotic use is essential for infection control. From this perspective, the objective of this research was to cultivate the microalgae Chlorella minutissima under batch regime, to evaluate the chlorophyll production and the antimicrobial activity of the microalgal oil on the S. aureus microorganism. The microalgae was cultivated for 33 days under constant light and aeration, with temperatures ranging from 21 ° C to 33 ° C. At the end of this period, the crop was flocculated and vacuum filtered to obtain dry biomass. The oil was obtained by the hot extraction method and its antimicrobial activity against Staphylococcus aureus bacteria was evaluated. As a result, the microalgae grew during the first 15 days of cultivation, when it entered the stationary phase. However, it achieved higher chlorophyll production on the 18th day of cultivation. The oil and extracts obtained from microalgae had a positive effect on growth inhibition of Staphylococcus aureus. Thus, although in-depth studies are required, the results suggest that bioproducts extracted from Chlorella minutissima microalgae are promising for the treatment of atopic dermatitis.
Objective: The aim of the present study was to verify the Hygienicsanitary aspect of artisanal and industrial ice creams and antimicrobial resistance in Escherichia coli isolated. Methods: 27 ice cream samples were purchase, detecting total coliforms at 30-35 °C by fermentation in multiple tubes. The tubes with positivity were inoculated in EC Broth and SS agar for the detection of Escherichia coli and Salmonella spp, respectively. To antimicrobial tests, plaque diffusion technique was used, discriminating levels of resistance. Genetic variability was diagnosed by the PCR-RAPD and ERIC-PCR techniques through the polymorphic degree determined by the band pattern. Results and Discussion: From the microbiological samples, there was positivity for E. coli (48%) and Salmonella spp (22%). The antimicrobial resistance tests in E. coli diagnosed resistance to 85% of the samples obtained, whereas 34% were multiresistant. A high degree of polymorphism was found in the PCR-RAPD and ERIC-PCR tests, demonstrating the great genetic variability between the bacteria. Conclusion: The samples presented unsatisfactory conditions, offering hazards to the consumers' health. The antibiogram test expressed resistance and multiresistance, while the PCR-RAPD and ERIC-PCR tests indicated great genetic variability among the bacteria, considering multiple sources of contamination. Therefore, measures to control contamination prevention are considered important.
Produção de biomassa da microalga Chlorella minutissima usando mel como fonte de carbono Biomass production of microalga Chlorella minutissima using honey as carbon source
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