Background/Aim. There is limited published evidence on the cytotoxicity of 3D printed polymer materials for dentistry applications, despite that they are now being widely used in medicine. Stereolithography (SLA) is one of the foremost 3D processes used in 3D printing, yet there are only a small number of resin materials reported to be suitable for medical applications. The aim of this study was to investigate, in vitro, the cytotoxic effect of the 3D printed resin in order to establish the suitability for its usage in dentistry and related medical applications such as surgical dental guides, occlusal splits and orthodontic devices. Methods. To examine the cytotoxicity of the 3D printed polymer-based epoxy resin, Accura? ClearVue? (3D-Systems, USA), two cell cultures were used: mouse fibroblasts L929 and human lung fibroblasts MRC-5. Cell viability was determined by Mosmann's colorimetric (MTT) test and the agar diffusion test (ADT). Results. Direct contact of the tested material with ADT test showed nontoxic effects of tested material in any cell culture. The tested material showed no cytotoxic effect after 3 days of extraction of the eluate by MTT test, but mild cytotoxic effect after 5, 7 and 21 days on both cell lines. The cytotoxicity increased with increasing the time of the eluate extraction. Conclusion. 3D printed polymer-based epoxy resin, Accura? ClearVue? (3D-Systems, USA) is considered appropriate for making surgical dental implant guides according to the cytotoxic behavior. According to the mild level of cytotoxicity after the longer extraction periods, there is a need for further evaluation of biocompatibility for its application for occlusal splints and orthodontic devices. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. TR 35020: Research and development of modeling methods and approaches in manufacturing of dental recoveries with the application of modern technologies and computer aided systems]
The temporomandibular joint (TMJ) represents a biomechanically and morphologically complex structure, tightly connected with the development and growth of mandible and craniofacial complex. The aim of this article is to comprehensively present contemporary diagnostic modalities and clinical implications for imaging of the TMJ. Contemporary imaging modalities, if used properly and according to adequate clinical implications and criteria, are able to depict different pathological processes and play a crucial role in establishing the right diagnosis and monitoring therapeutic effect. The key to right diagnosis, however, still lies in good knowledge of the TMJ developmental and functional anatomy, as well as the TMJ dysfunction related to the jaws, surrounding muscles, teeth, and cranial base.
Introduction. Saliva plays an important role in maintaining oral health. Its buffer capacity helps neutralise acidic products from food, drinks and dental plaque. Saliva composition, quantitative and qualitative properties, such as acidity, are associated with the occurrence of dental caries, non-carious lesions and periodontitis. The aim of the study was to examine the change in salivary acidity after taking carbonated mineral water and sugar-sweetened carbonated soda in subjects aged 18 to 25 years. Materials and method. The study research was conducted at the Dentistry Clinic of Vojvodina on 30 adult subjects. Salivary acidity was measured with a digital pH meter before, and 5, 10 and 20 minutes after taking 200 ml of carbonated mineral water and 200 ml of sugar-sweetened carbonated soda. The obtained results were statistically processed by mixed, combined analysis of variance. Results. Consumption of carbonated mineral water and sugar-sweetened carbonated soda led to a change in salivary acidity in the examined time intervals. Carbonated mineral water reduced the salivary acidity, and after 20 minutes the acidity recovers reaching levels close to the initial pH value. After taking sugar-sweetened carbonated soda, the salivary pH value dropped. It reached the lowest mean value 5 and 10 minutes after taking the beverage, and then increased slightly without reaching the initial value. In some subjects, the minimum value of the salivary pH obtained was below the value considered critical for the occurrence of enamel and dentin demineralisation. Conclusion. Carbonated mineral water and sugar-sweetened carbonated soda affected the salivary pH value within a period of 20 minutes after consumption. Consuming sugar-sweetened carbonated soda can have adverse effects on oral health.
Introduction. Tooth color can be represented in a three-dimensional color system by applying L* (lightness), a* (red-green color tone) and b* (yellow-blue color tone) coordinates. Clinically significant color difference, Delta-E, can be expressed in units that are correlated with visual perception using the International Commission on Illumination L*a*b* system. The aim of the study was spectrophotometric analysis of the natural maxillary central incisor color, and to establish any gender-related differences in lightness, red-green tone, and yellow-blue tone coordinates. Material and Methods. The study included 80 students (40 male and 40 female, with an average age of 22 years). The color of the maxillary right central incisor was determined using an intraoral spectrophotometer and VITAPAN Classical and VITA 3D-Master color shade guides. Lightness, red-green tone, and yellow-blue tone values were recorded for each result. The obtained data were processed by using ?2, Student?s t-test and Mann-Whitney test. Results. Results showed no statistically significant gender-related difference in color according to the VITApan Classical and VITA 3D-Master color key. No difference was found in the lightness of the central maxillary incisor between genders, or in terms of red-green tone (p = 0.860573). A statistically significant difference was found when observing the sex-related yellow-blue tone of the color of the central maxillary incisor. Conclusion. The analysis of colorimetric parameters of the International Commission on Illumination L*a*b* system provides useful information about the tooth color in the examined population, while potentially revealing differences in lightness, red-green tone and yellow-blue tone between genders.
The pro-apoptotic effect of new 2-thioxo-4-thiazolidinone derivative Les-3331 on MCF-7 and MDA-MB-231 cell lines ..
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.