The aim of the study was to assess the effects of
training on haematological and biochemical blood parameters as well as on
the changes in body surface temperature in horses. In order to identify the
predictive value of surface temperature measurements as a marker of animal's
performance, their correlations with blood parameters were investigated. The
study was carried out on nine horses divided into two groups: routinely ridden
and never ridden. Infrared thermography was used to assess surface
temperature changes before (BT) and just after training (JAT) on a
treadmill. Seven regions of interest (ROIs) located on the neck, shoulder,
elbow, back, chest, gluteus and quarter were analysed. The blood samples
were taken BT, JAT and 30 min after training (30AT). Haematological
parameters including white blood cells, lymphocytes (LYMs), monocytes (MONOs),
granulocytes (GRAs), eosinophils (EOSs), haematocrit (HCT) and platelets (PLTs)
as well as biochemical parameters such as glucose (GLUC), urea,
chemnormalNa+, chemnormalK+ and chemnormalCa2+, and creatine phosphokinase (CPK) were
analysed. Our results indicated a significant increase in surface
temperature JAT (p=0.043) in the neck, shoulder, elbow, gluteus and
quarter in routinely ridden horses. Significant changes in EOS (p=0.046)
and HCT (p=0.043) in the case of the never-ridden and routinely ridden group,
respectively, were found between the times of blood collection. In addition,
there was a significant effect of the horse group and the time of blood
collection on the CPK activity (p=0.025 to p=0.045) and urea
concentrations (p=0.027 to p=0.045). In the routinely ridden horses,
there were significant correlations between the changes in MONO
(ρ=0.40), GRA (ρ=-0.40), PLT (ρ=-0.77), HCT (ρ=-0.36), GLUC
(ρ=0.56) and urea (ρ=0.56) and the total ROI temperature changes.
Moreover, significant correlations between the changes in MONO
(ρ=-0.86), EOS (ρ=-0.65), GLUC (ρ=0.85), urea (ρ=0.85),
chemnormalNa+ (ρ=0.59) and chemnormalK+ (ρ=-0.85) and the total ROI
temperature changes were found in never-ridden horses. Different changes in
body surface temperature and blood parameters in routinely ridden and
never-ridden horses could be associated with different conditioning and
performance. A significantly higher surface temperature in routinely ridden
horses, as well as the dynamics of changes in HCT, CPK and urea after
training indicate better performance of these horses. Significant
correlations between MONO, GLUC, and urea and a total ROI surface temperature
as well as a negative correlation between MONO and the total ROI temperature
in never-ridden horses indicated poor performance.