No abstract

The size of oocytes during germinal vesicle migration (GVM) and germinal vesicle breakdown (GVBD) was revised for the Leopard Grouper Mycteroperca rosacea. Histology of ovaries, plasma levels of estradiol‐17ß (E2), and testosterone (T) were analyzed in Leopard Grouper at 0, 12, 16, and 24 h after a priming dose of 1,000 IU/kg body weight of human chorionic gonadotropin (hCG) and were compared with control females injected with saline solution. Histological observations were verified in hormonally induced females by observing in vivo intraovarian biopsies after using the same priming dose and a second resolving dose of 500 UI/kg body weight of hCG 16 or 24 h later, which served to analyze spawning performance. Histological and in vivo intraovarian biopsies showed that in full‐yolk oocytes (~455 μm diameter) GVM and GVBD were concomitant with coalescence of a single oil droplet 16 and 24 h after the first injection. The oil droplet became a morphological marker as well as a predicting indicator of the competence and synchronization of maximum vitellogenic oocytes and the effectiveness to respond to hormonal therapy. In the experimental fish group E2 and T reached peaks at the same time, which were significantly higher than those in the controls. Mean fecundity was 168,000 eggs/kg, mean fertilization was 88%, and mean viability was 71% (114,000 eggs/kg), all of which were within the range of other cultivated groupers. These results can be used to improve the induction of final maturation in the Leopard Grouper in captivity and may be used in other species of Mycteroperca that show the same marker.

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