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We investigated the bulk electrical conductivity and microbial population distribution in sediments at a site contaminated with light nonaqueous-phase liquid (LNAPL). The bulk conductivity was measured using in-situ vertical resistivity probes; the most probable number method was used to characterize the spatial distribution of aerobic heterotrophic and oil-degrading microbial populations. The purpose of this study was to assess if high conductivity observed at aged LNAPLimpacted sites may be related to microbial degradation of LNAPL. The results show higher bulk conductivity coincident with LNAPL-impacted zones, in contrast to geoelectrical models that predict lower conductivity in such zones. The highest bulk conductivity was observed to be associated with zones impacted by residual and free LNAPL. Data from bacteria enumeration from sediments close to the resistivity probes show that oil-degrading microbes make up a larger percentage (5-55%) of the heterotrophic microbial community at depths coincident with the higher conductivity compared to ∼5% at the uncontaminated location. The coincidence of a higher percentage of oil-degrading microbial populations in zones of higher bulk conductivity suggests that the higher conductivity in these zones may result from increased fluid conductivity related to microbial degradation of LNAPL, consistent with geochemical studies that suggest that intrinsic biodegradation is occurring at the site. The findings from this study point to the fact that biogeochemical processes accompanying biodegradation of contaminants can potentially alter geoelectrical properties of the subsurface impacted media.

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Sixty leading members of the scientific, engineering, regulatory, and legal communities assembled for the PFAS Experts Symposium in Arlington, Virginia on May 20 and 21, 2019 to discuss issues related to per‐ and polyfluoroalkyl substances (PFAS) based on the quickly evolving developments of PFAS regulations, chemistry and analytics, transport and fate concepts, toxicology, and remediation technologies.  The Symposium created a venue for experts with various specialized skills to provide opinions and trade perspectives on existing and new approaches to PFAS assessment and remediation in light of lessons learned managing other contaminants encountered over the past four decades. The following summarizes several consensus points developed as an outcome of the Symposium: Regulatory and policy issues: The response by many states and the US Environmental Protection Agency (USEPA) to media exposure and public pressure related to PFAS contamination is to relatively quickly initiate programs to regulate PFAS sites. This includes the USEPA establishing relatively low lifetime health advisory levels for PFAS in drinking water and even more stringent guidance and standards in several states. In addition, if PFAS are designated as hazardous substances at the federal level, as proposed by several Congressional bills, there could be wide‐reaching effects including listing of new Superfund sites solely for PFAS, application of stringent state standards, additional characterization and remediation at existing sites, reopening of closed sites, and cost renegotiation among PRPs. Chemistry and analytics: PFAS analysis is confounded by the lack of regulatory‐approved methods for most PFAS in water and all PFAS in solid media and air, interference with current water‐based analytical methods if samples contain high levels of suspended solids, and sample collection and analytical interference due to the presence of PFAS in common consumer products, sampling equipment, and laboratory materials. Toxicology and risk: Uncertainties remain related to human health and ecological effects for most PFAS; however, regulatory standards and guidance are being established incorporating safety factors that result in part per trillion (ppt) cleanup objectives. Given the thousands of PFAS that may be present in the environment, a more appropriate paradigm may be to develop toxicity criteria for groups of PFAS rather than individual PFAS. Transport and fate: The recalcitrance of many perfluoroalkyl compounds and the capability of some fluorotelomers to transform into perfluoroalkyl compounds complicate conceptual site models at many PFAS sites, particularly those involving complex mixtures, such as firefighting foams. Research is warranted to better understand the physicochemical properties and corresponding transport and fate of most PFAS, of branched and linear isomers of the same compounds, and of the interactions of PFAS with other co‐contaminants such as nonaqueous phase liquids. Many PFAS exhibit complex transport mechanisms, particular...

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A newly proposed geoelectrical model for hydrocarbon contaminated sites predicts high conductivities coincident with the contaminated zone as opposed to the traditionally accepted low conductivity. The model attributes the high conductivities to mineral weathering resulting from byproducts of microbial redox processes. To evaluate this conductive model, in situ vertical conductivity measurements were acquired from a light non‐aqueous phase liquid (LNAPL) contaminated site. The results showed high conductivities coincident with the zone of contamination and within the smear zone influenced by seasonal water table fluctuations. We infer this zone as an active zone of biodegradation and suggest significant microbial degradation under partially water saturated conditions. A simple Archie's Law analysis shows large pore water conductivities necessary to reproduce the bulk conductivity measured at the contaminated location. This study supports the conductive layer model and demonstrates the potential of geoelectrical investigations for assessing microbial degradation of LNAPL impacted soils.

Bulk electrical conductivity of sediments during microbial mineralization of diesel was investigated in a mesoscale laboratory experiment consisting of biotic contaminated and uncontaminated columns. Population numbers of oil degrading microorganisms increased with a clear pattern of depth zonation within the contaminated column not observed in the uncontaminated column. Microbial community structure determined from ribosomal DNA intergenic spacer analysis showed a highly specialized microbial community in the contaminated column. The contaminated column showed temporal increases in bulk conductivity, dissolved inorganic carbon, and calcium, suggesting that the high bulk conductivity is due to enhanced mineral weathering from microbial activity. The greatest change in bulk conductivity occurred in sediments above the water table saturated with diesel. Variations in electrical conductivity magnitude and microbial populations and their depth distribution in the contaminated column are similar to field observations. The results of this study suggest that geophysical methodologies may potentially be used to investigate microbial activity.

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