Biomedical sciences, and in particular biomarker research, demand efficient glycoprotein enrichment platforms. Herein magnetic nanoprobes (MNP), after being coated with three broad-spectrum lectins-concanavalin A (ConA), wheat germ agglutinin (WGA), and Maackia amurensis lectin (MA)-were utilized to selectively capture glycoproteins from human body fluids. Additionally, a new methodology, based on protection of the lectins with their target sugars prior to coupling with MNPs, was proposed to overcome the nonspecific nature of conjugation. This approach contributed to preserve lectin conformation, increasing by 40% and 90% the affinity of ConA and MA for glycoproteins in relation to synthesis with nonprotected lectins. Optimal operating conditions (temperature, time) and maximum binding capacities were further determined for each lectin by use of fetuin as a reference. The enhanced performance of lectin-based nanoplatforms was demonstrated by comparing MNP@ConA with conventional Sepharose@ConA. These experiments have shown that ConA immobilized on MNP exhibited 5 times higher affinity for fetuin and ovalbumin when compared with Sepharose@ConA with the same amount of immobilized lectin. MNP@Lectins were then applied to human serum, saliva, and urine and the recovered proteins were digested with trypsin and analyzed by nano-HPLC MALDI-TOF/TOF. This allowed the identification of 180 proteins, 90% of which were found to be glycosylated by use of bioinformatics tools, therefore revealing low levels of unspecific binding. Thus, MNP@lectins have proved to be a valuable tool for glycoproteomic studies, particularly when dealing with minute amounts of material.

The overall purpose of this study was to examine the mediating effects of parents' coping strategies on the relationship between parents' emotional intelligence and sideline verbal behaviors during their children's soccer games. Participants were 232 parents (120 mothers and 110 fathers) of youth soccer players age 9-13 years. Observations in situ were carried out at 30 soccer games during a soccer tournament. At the end of the game, parents were approached and asked to complete the Emotional Intelligence Scale and the Brief COPE scale. Structural-equation-modeling analyses revealed that adaptive and maladaptive coping mediated the relationship between regulation of emotion and parents' praise/encouragement, and negative and derogatory comments during the game. In addition, game result moderated the relationships between emotional intelligence, coping strategies, and parent behaviors. Emotional regulation and adaptive coping may promote desirable parent sideline behaviors and reduce undesirable behaviors.

Knowledge of players’ anthropometric profiles may contribute to a better understanding of the differences between their performance levels. For example, vertical jump height, which is a major indicator of a volleyball player’s performance, is influenced by favorable anthropometric variables. This study’s aim was to describe anthropometric profiles of elite female volleyball players competing in the 1st and 2nd divisions in Portugal and to link these profiles with performance skills such as jump height obtained during blocking and spiking actions. Fifty-nine volleyball players were analyzed according to their competitive level, forming three independent groups: GA (n = 20, A1 division, ranked first), GB (n = 21, A1 division, ranked last) and GC (n = 18, A2 division). Anthropometric data collected included body mass and height, arm span, seven skinfolds (triceps, biceps, subscapular, suprailiac, abdominal, thigh and calf), four body perimeters (relaxed brachial, contracted brachial, thigh and calf), and two body diameters (humeral and femoral). Performance data included the height obtained during block and spike actions. Significant differences were found between groups (GA/GB from A1 and GC from A2). Players in the GA had the highest body mass (68.05 ± 6.62 kg, p < 0.05), body height (176.35 ± 6.21 cm, p < 0.05), arm span (177.59 ± 6.09 cm, p < 0.05), lean mass (53.51 ± 4.94 kg, p < 0.05) and vertical jump heights (block: 0.36 ± 0.06 m and spike: 0.43 ± 0.05 m, p < 0.05). As expected, the fat mass percentage of GA players was the lowest (21.30 ± 2.61%). The results suggest that anthropometric profiles of volleyball players may vary according to the competitive level. The higher body mass, body height, arm span, and lean mass presented by GA players in comparison with GC players suggest these variables are important for top-level performance, since these athletes also exhibited higher jump heights. Variables such as height and arm span have a considerable genetic influence and could be important for early talent identification in volleyball. Other variables such as body mass, lean mass and vertical jump performance are more complex, since they also reflect the effects of environmental and training conditions.

The higher Nash blowup of an algebraic variety replaces singular points with limits of certain spaces carrying higher-order data associated to the variety at non-singular points. In this note we will define a higherorder Jacobian matrix that will allow us to make explicit computations concerning the higher Nash blowup of hypersurfaces. Firstly, we will generalize a known method to compute the fiber of this modification. Secondly, we will give an explicit description of the ideal whose blowup gives the higher Nash blowup. As a consequence, we will deduce a higher-order version of Nobile's theorem for normal hypersurfaces. * Research supported by CONACyT (México) through the program Cátedras para Jóvenes Investigadores.

We perform molecular dynamics simulations of tetraalkylammonium ionic liquids confined in the interlayer of montmorillonite (MMT). We study the structure and energetics of the systems, which consist of cations with two different alkyl chain lengths and several ionic liquid concentrations. The results we obtained for the structure, namely the presence of a strong layering in all systems and the formation of nonpolar domains with interdigitated alkyl chains in some cases, are largely consistent with previous surface force balance experiments performed on similar systems. Finally, we show that swelling of the organo-modified MMT by a large amount of ionic liquid seems energetically favorable in all cases.