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One of the main components for being successful in tissue engineering is developing a scaffold with an appropriate architecture for allow migration, cell proliferation, and differentiation. A gelatinchitosan scaffold by vacuum freeze-drying has been developed for tissue engineering applications. The effects of solid concentration and freezing processing on the scaffold morphology and porous size were investigated. As the chitosan content was increased the viscoelastic properties of pigskin gelatin was modified, the maximum G' values were lower than the values for pure gelatin solution, and the thermal transition points also occurred at lower temperatures, as well as a decrease of pore size tendency was observed and the scaffold visibly increased porosity, the structure scaffold was observed with an interconnected and more homogeneous pore matrix. The pore sizes become smaller and pore walls thinner, while interconnectivity increases along with declining pre-freezing temperature. The chitosan-gelatin scaffold will be a promising candidate in tissue engineering.

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The purpose of this quantitative study was to analyze the effects of close reading instruction on students' reading comprehension. Close reading is an instructional strategy that allows students to dig deep into the reading and gain a profound understanding of the vocabulary, writing structure and author's message. The study consisted of a total of twenty-one 6th grade students who received close reading as an experimental treatment. The treatment included several components of close reading: essential questions, graphic organizers to compare short non-fiction stories, written analysis of author's point of view/theme and weekly tests. The my master's degree, provided me with mentorship, constructive feedback and endless editing on my thesis. My 6th grade class (2017-18) who allowed me to use them in my research study. They were the true champions! Finally, my family for always supporting me, but especially to my brother Alberto who helped me refine my thoughts and research v

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