Abstract. A stable pressure driving system was designed in this paper, which can be used in Microfluidic Chips to driving reagents. Herein, two embedded controller units are used to control the stability of the reagent bottles pressure, for driving the reagents flow steady on the microfluidic chips. All parameters of system can be set by a friendly upper computer interface, and the upper and lower computer interactive communication via Wi-Fi. Y-junction microfluidic chip was taken to generate water-in-oil droplets that can be observed by Microfluidic Optical Detection Platform. Through the reagent bottles pressure control waveforms and the distribution of droplets sizes to verify the stability and reliability of stable pressure driving system. This convenient and practical system can be widely used in the reagents driving of Microfluidic Chips, and easy to implement automation may one day help enable the realization of the intelligent laboratory.
Patients infected human papillomavirus (HPV) is increasing all over the world, in particular the community of woman. Microarray-in-a-tube system is a novel method of integrating polymerase chain reaction (PCR) with the microarray technique and a simple and high-throughput detection system. In this work, the microarray-in-a-tube system used in the function of detection human papillomavirus genotypes. Detection human papillomavirus genotypes of a microarray-in-a -tube system was composed of the preparation arrays, PCR, hybridization on chip, fluorescence detection, containing general GP5+/GP6+ premier, 30 targets. These results suggest that signal-noise ratio (SNR) of this system was set as SNR ≥ 2.0 at 100 copies. The system was suitable for other disease diagnosis in future.
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