Objective Vascular precursor cells with angiogenic potentials are important for tissue repair, which is impaired in diabetes mellitus. MicroRNAs are recently discovered key regulators of gene expression, but their role in vascular precursor cell–mediated angiogenesis in diabetes mellitus is unknown. We tested the hypothesis that the microRNA miR-27b rescues impaired bone marrow–derived angiogenic cell (BMAC) function in vitro and in vivo in type 2 diabetic mice. Approach and Results BMACs from adult male type 2 diabetic db/db and from normal littermate db/+ mice were used. miR-27b expression was decreased in db/db BMACs. miR-27b mimic improved db/db BMAC function, including proliferation, adhesion, tube formation, and delayed apoptosis, but it did not affect migration. Elevated thrombospondin-1 (TSP-1) protein in db/db BMACs was suppressed on miR-27b mimic transfection. Inhibition of miR-27b in db/+ BMACs reduced angiogenesis, which was reversed by TSP-1 small interfering RNA (siRNA). miR-27b suppressed the pro-oxidant protein p66shc and mitochondrial oxidative stress, contributing to its protection of BMAC function. miR-27b also suppressed semaphorin 6A to improve BMAC function in diabetes mellitus. Luciferase binding assay suggested that miR-27b directly targeted TSP-1, TSP-2, p66shc, and semaphorin 6A. miR-27b improved topical cell therapy of diabetic BMACs on diabetic skin wound closure, with a concomitant augmentation of wound perfusion and capillary formation. Normal BMAC therapy with miR-27b inhibition demonstrated reduced efficacy in wound closure, perfusion, and capillary formation. Local miR-27b delivery partly improved wound healing in diabetic mice. Conclusions miR-27b rescues impaired BMAC angiogenesis via TSP-1 suppression, semaphorin 6A expression, and p66shc-dependent mitochondrial oxidative stress and improves BMAC therapy in wound healing in type 2 diabetic mice.
IntroductionGestational diabetes mellitus (GDM) is one of the most prevalent diseases during pregnancy, which is closely associated with many short-term and long-term maternal and neonatal complications and can incur heavy financial burden on both families and society. Web-based interventions have been used to manage GDM because of the advantages of high accessibility and flexibility, but their effectiveness has remained inconclusive. This systematic review and meta-analysis aims to comprehensively investigate the multidimensional effectiveness of web-based interventions for pregnant women with GDM, thereby aiding implementation decisions in clinical settings.Methods and analysisThis systematic review protocol strictly adheres to the Preferred Reporting Items for Systematic Review and Meta-Analysis Protocols guidelines. Six electronic databases (PubMed, Web of Science, Cochrane Central Register of Controlled Trials, Embase, CINAHL and PsycINFO) will be comprehensively searched from their inception to 26 January 2022 to identify randomised controlled trials and controlled clinical trials regarding the efficacy of web-based interventions for pregnant women with GDM on glycaemic control, behavioural outcomes, cognitive and attitudinal outcomes, mental health, maternal and neonatal clinical outcomes, and medical service utilisation and costs. Two reviewers will independently conduct the study selection, data extraction and quality assessment. The methodological quality of included studies will be assessed using the Effective Public Health Practice Project assessment tool. The overall meta-analyses for each of the interested outcomes will be performed if the outcome data are sufficient and provides similar effect measures, as well as subgroup analyses for glycaemic control indicators based on the different types of intervention format, interactivity and technology. We will conduct a qualitative synthesis for studies that cannot be quantitatively synthesised.Ethics and disseminationEthics approval is not required for this review as no human participants will be involved. The results will be disseminated via a peer-reviewed journal or an academic conference.PROSPERO registration numberCRD42022296625.
Compared with minimal quantity lubrication (MQL), electrostatic atomization can control the movement of mist droplets by changing the electrostatic field, thus reducing the drift of mist droplets in air. This paper first proposes the concept of electrostatic atomization cutting, and then develops a 3D FE model of electrostatic field for electrostatic atomization cutting using a commercial software Ansoft Maxwell. By simulation, the influence of nozzle angle, structure, and electrode gap and voltage on electric field intensity is investigated. Based on simulation results, the optimal nozzle angle and structure are obtained for electrostatic atomization cutting. The findings contribute to further development of this technique.
On the basis of conventional level casting, low frequency electromagnetic field is applied during conventional level casting process. Effects of low frequency electromagnetic field on melt flow, macrostructure and microstructure have been studied. The experiment is performed on the Al-4.5% Cu alloy, and the experimental results show that with the application of the low frequency electromagnetic field, the macrostructure and microstructure are changed greatly. The reasons for the low frequency electromagnetic field changing the macrostructure and microstructure of the ingot were summarized.
The effects of the low frequency electromagnetic field on the macrosegregation of the 7075 aluminum ingots were investigated. The 7075 aluminum ingots with the diameter of 200 mm were prepared by the conventional direct chill casting and the low frequency electromagnetic field casting (LFEC) processes, respectively. The temperature during casting at steady state was measured, and the mushy region was observed from the temperature contour. The concentrations of the alloying elements were measured by the spectrograph. It was found that the transition region was broadened, but the mushy zone became narrower with presence of the low frequency electromagnetic field. The centerline macrosegregation of the ingots was alleviated by the low frequency electromagnetic casting process.
Objective This study aims to evaluate the efficacy and safety of oxycodone hydrochloride (OxyContin) rectal administration in cancer pain patients. This is geared towards providing the research evidence for a novel route of OxyContin administration. Methods Relevant randomized controlled trials (RCTs) were searched in electronic databases, including PubMed, Cochrane Library, Web of Science, EMBASE, China National Knowledge Infrastructure (CNKI), Chinese Scientific Journal Database (VIP database), Wanfang Data Knowledge Service Platform, and Chinese Biomedical Literature Database (CBM). Moreover, unpublished academic data were obtained by contacting the colleague, professor, or Institute of Traditional Chinese Medicine. The RCTs of transrectal Oxycodone administration of sustained-release tablets for moderate and severe pain patients were searched in the databases from inception to December 2020. Results According to the inclusion criteria, a total of 8 RCTs were included, with a total of 648 patients. Meta analysis results showed that there was no statistically significant difference in the efficacy of moderate to severe pain control between the rectal administration group and the oral administration group (RR = 1.04, 95%CI: 0.99–1.10, p = 0.13>0.05). At the same time, the incidence of adverse reactions in the rectal administration group was low. In terms of constipation, the rectal administration group was less than the oral administration group, with a statistically significant difference (RR = 0.43, 95%CI: 0.31–0.58, p< 0.00001). In terms of nausea and vomiting, the rectal administration group was less than the oral administration group, and the difference was statistically significant(RR = 0.30, 95%CI: 0.21–0.42, p<0.00001). In terms of sleepiness, there was no significant difference between the two groups(RR = 0.54, 95%CI: 0.26–1.15, p = 0.11>0.05). In terms of dizziness, there was no statistically significant difference between the two groups (RR = 0.43, 95%CI:0.27–0.68, p = 0.31>0.05). In terms of dyuria, there was no statistically significant difference between the two groups (RR = 0.37, 95%CI: 0.02–7.02, p = 0.51>0.05). In terms of KPS scores there was no significant difference was noted between the rectal and oral administration groups (RR = 1.04, 95%CI: 0.89–1.21, p = 0.63>0.05). Conclusion In summary, we found no significant differences in efficacy between rectal administration of OxyContin and oral administration. Thus, rectal administration should be considered in managing cancer pain among patients with difficulty in oral OxyContin administration. PROSPERO registration number CRD42021209660.
Al-4.5%Cu ingots were prepared by a process of low frequency electromagnetic casting (LFEC) and conventional direct chill (DC) casting, respectively. The effects of low frequency electromagnetic field on the microsegregation were investigated from eutectic analysis and electro probe microanalysis (EPMA). It was found that the amount of the nonequilibrium eutectic and the dimension of the nonequilibrium eutectic were decreasing markedly. In contrast, the solute content in the α-Al phase increased to a certain extent in the presence of the low frequency electromagnetic field, and it increased with the incerasing electromagnetic.
What the paper describes is to develop the performance calculation program and it will be used in the practical unit through analyzing the thermal process in the power plant, which is on the basis of the operation performance of the power plant equipment and the analysis of the calculation method in accordance with the first law of thermodynamics. We can be analyzed through calculating and quantifying the performance of the whole boiler, the turbine, the thermodynamic system and the power plant in detail. The operation quality and the intact degree of unit equipment can be evaluated by the operation personnel according to performance data. Finally, it is going to save energy reduce consumption, and realize the goal of improving level of the unit operation.
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