Introduction: Pyroptosis induced by lipopolysaccharide (LPS) is a dissolved form of cell death. The molecular marker gasdermin D, specifically GSDMD-N, is critically required for the induction of pyroptosis. Recently, there have been studies showing that LPS is closely related to tumor biology. Methods: Specimens from 40 patients with colorectal cancer (CRC) were collected. Eight-to twelve-week-old C57BL6 male mice (n=30) were raised. Immunohistochemistry and Western blot were performed to test the expression of GSDMD. Moreover, cytotoxicity assay, IL-18 and IL-1β ELISA, Annexin V and PI stain, and wound healing assay were also made. Gene Expression Profiling Interactive Analysis (GEPIA) was used to verify the expression of GSDMD and overall survival of CRC patients with a high/low expression of GSDMD. Results: In the research, we showed that the poor prognosis in CRC patients was significantly related to the GSDMD expression and significantly down-regulated in human colorectal cancer (CRC) tissues. Treatment with LPS, but not TNF-α, induced pyroptosis via promoting the expression of GSDMD and GSDMD-N membrane translocation and enhanced chemosensitivity in response to L-OHP in HT29 cells. Furthermore, the enforced expression of GSDMD in HT29 cells reduced cell survival and induced cell death. Discussion: These results of studies suggest that the low expression of GSDMD correlates with a poor CRC prognosis, and that pyroptosis induced by LPS may improve the anti-cancer effect of L-OHP, inhibiting the tumorigenesis of CRC by activating GSDMD. Our findings lay the foundation for further development of GSDMD serving as an important prognostic biomarker and a valid CRC therapeutic target.
The aluminium alloy bar is mainly obtained by hot extrusion in the past. In order to increase the production efficiency and reduce the cost, a new mold is designed, and horizontal continuous casting is used to produce the aluminium alloy bar in this work. The results show that aluminium alloy bar can be cast by horizontal continuous casting. The asymmetry of the sump is overcome by optimizing the design of the mold. When the temperature of the melt was 730°C, casting speed is 650 mm/min, cooling water is 15 L/min, the quality of lubricating oil is 0.5 ml/min, and the ingot with good surface quality can be cast.
7075 aluminum alloy ingot with the diameters of 100 mm was produced by Horizontal Direct-Chill (HDC). The temperature in the melt was measured and the ingots were examined in detail with the aim to reveal the floating grains in the HDC ingot. Experimental results show that very large floating grains with coarse dendrite arm spacing (DAS) mainly concentrate in the half-moon area near bottom surface of the HDC casting ingot, under the function of gravity. The floating grains in the HDC casting process is much bigger than that founded in Vertical Direct-Chill (VDC) casting process.
The effect of increasing the amount of added grain refiner and combination electromagnetic filed on grain size and morphology has been investigated by die casting of commercial aluminum. The results show the grain size and the grain morphology both are transited with the increasing of the addition of Al-5Ti-B. The grain size continuously decreased with increasing the addition of grain refiner. Without grain refiner additions, an obvious transition in grain size and morphology is observed with the application of low frequency electromagnetic field (LFES). The efficiency in grain refinement of LFES decreases with increasing the amount of grain refiner. However, even when the addition of grain refiner is 2 wt. % Al-5Ti-B, 63 % grain size reduction still is observed by the affect of LFES. This result shows that the LFES can enhance the efficiency in grain refinement of grain refiner.
The rules of the distribution of magnetic field were carried out by numerical simulation. The distribution of magnetic field was got, and the effects of current intensity and frequency on the distribution of magnetic field were analyzed by constructing three-dimensional finite element model and using ANSYS software which is a kind of commercial FEM analysis software. The results show that the intensity of magnetic field is proportional to current intensity, magnetic field is mainly localized in the surface region of liquid melt and there is a notable edge effect in the corner.
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