ABSTRACT:We captured 36 Northern Bobwhites (Colinus virginianus) in Mitchell County, Texas in June-September 2013, and examined them for the eyeworm Oxyspirura petrowi. We recovered 334 eyeworms from 28 of 29 adult bobwhites (97%); infections ranged from 1-40 worms and mean (6SD) abundance of 11.9613.0. Three of seven juveniles were infected, and those infected had one eyeworm each. Prevalence of eyeworms was similar among months. However, mean abundance of eyeworms peaked in July and August (3.362.1, 13.5615.0, and 16.9615.5), and decreased in September (6.363.0). We suggest that several previous studies may underreport prevalence and abundance because in those studies only the eye surface and nictitating membrane were examined, and not eye-associated tissue, ducts, glands, or sinuses.

Wild populations of northern bobwhites (Colinus virginianus; hereafter bobwhite) have declined across nearly all of their U.S. range, and despite their importance as an experimental wildlife model for ecotoxicology studies, no bobwhite draft genome assembly currently exists. Herein, we present a bobwhite draft de novo genome assembly with annotation, comparative analyses including genome-wide analyses of divergence with the chicken (Gallus gallus) and zebra finch (Taeniopygia guttata) genomes, and coalescent modeling to reconstruct the demographic history of the bobwhite for comparison to other birds currently in decline (i.e., scarlet macaw; Ara macao). More than 90% of the assembled bobwhite genome was captured within <40,000 final scaffolds (N50 = 45.4 Kb) despite evidence for approximately 3.22 heterozygous polymorphisms per Kb, and three annotation analyses produced evidence for >14,000 unique genes and proteins. Bobwhite analyses of divergence with the chicken and zebra finch genomes revealed many extremely conserved gene sequences, and evidence for lineage-specific divergence of noncoding regions. Coalescent models for reconstructing the demographic history of the bobwhite and the scarlet macaw provided evidence for population bottlenecks which were temporally coincident with human colonization of the New World, the late Pleistocene collapse of the megafauna, and the last glacial maximum. Demographic trends predicted for the bobwhite and the scarlet macaw also were concordant with how opposing natural selection strategies (i.e., skewness in the r-/K-selection continuum) would be expected to shape genome diversity and the effective population sizes in these species, which is directly relevant to future conservation efforts.

In 2003 we conducted a study to determine the consequences of feral hog (Sus scrofa) invasions in several ecoregions of Texas. We examined the observations, experiences, and actions of landowners and managers concerning feral hogs on their property. We used purposive sampling of landowners and managers who fit 1 or more of 3 selection criteria. Landowners and managers were either sent a self‐administered, mail‐out questionnaire or given a copy of the questionnaire during pesticide applicator workshops. There were 775 survey participants. The effective response rate from those landowners and managers who received a mailed questionnaire was 62% (n=284). Nearly all (95%, n = 491) of the pesticide applicator workshop participants turned in a completed questionnaire. Sampling error based on the farms (includes ranches) in Texas and in each region was ±3%, α=0.05. The majority (74%) of respondents were ranchers, and 18% were farmers. Most respondents felt that feral hogs came from the neighbor's property and were an agricultural pest. Rooting, wallowing, and crop damage were the major forms of damage caused by feral hogs. The average economic loss due to hog damage, over the lifetime ownership of the land by the respondent, was $7,515 (U.S). Hog control was an incidental process. The average cost for hog control over the lifetime ownership of the land by the respondent was $2,631 (U.S.). There was strong support for programs related to feral hog management and control, but only half of the survey participants responded to the question. The average quiz score of 11.5 indicated that respondents could correctly respond to < 50% of the 26 questions. Region was found to have an effect (P < 0.05) on all questions tested except one. Management implications included the need for educational programs about feral hogs, how landowners can make better use of feral hogs on their property, ongoing education efforts about feral hogs, and the impact of this study on the public policy and decision‐making process.

The effects of Oxyspirura petrowi infections in northern bobwhites ( Colinus virginianus ) are not well understood. While studies have reported O. petrowi infections, none has histopathologically examined the eye surface and intraorbital glands to assess cellular-level impacts associated with infection. This study is the first to document the histopathology associated with O. petrowi infections. Oxyspirura petrowi occurred on the eye surface as well as in the conjunctiva, lacrimal ducts, lacrimal glands, and Harderian glands. Histopathology showed infections of O. petrowi caused cellular damage to these tissues, scarring and interstitial keratitis of the cornea, and acinar atrophy of the Harderian gland.

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Northern bobwhite (Colinus virginianus) and scaled quail (Callipepla squamata) have experienced chronic declines within the Rolling Plains ecoregion of Texas. Parasitic infection, which has long been dismissed as a problem in quail, has not been studied thoroughly until recently. A total of 219 northern bobwhite and 101 scaled quail from Mitchell County, Texas were captured and donated from 2014 to 2015, and examined for eyeworm (Oxyspirura petrowi) and caecal worm (Aulonocephalus pennula) infections. In 2014, bobwhites averaged 19.6 ± 1.8 eyeworms and 98.6 ± 8.2 caecal worms, and 23.5 ± 2.1 eyeworms and 129.9 ± 10.7 caecal worms in 2015. Scaled quail averaged 4.8 ± 1.0 eyeworms and 50 ± 6.8 caecal worms in 2014, and 5.7 ± 1.3 eyeworms and 38.1 ± 7.1 caecal worms in 2015. This study expands the knowledge of parasitic infection in quail inhabiting the Rolling Plains of Texas. A significant difference was documented in O. petrowi infection between species but there was no significant difference in A. pennula between quail species. No significant difference was detected in parasite infection between the sexes of both northern bobwhite and scaled quail. This study also documented the highest reported O. petrowi infection in both species of quail. Additional research is needed on the life history and infection dynamics of O. petrowi and A. pennula infections to determine if there are individual- and/or population-level implications due to parasitic infection.

The Northern Bobwhite ( Colinus virginianus ) has been steadily declining throughout much of its historic range for decades. The Rolling Plains ecoregion of Texas and western Oklahoma, historically rich with wild Northern Bobwhites and one of the last remaining quail strongholds, also has a declining population. During August and October in 2011-13, 348 Northern Bobwhites from the Rolling Plains were examined for eyeworms (Oxyspirura petrowi). Of these 348 Northern Bobwhites, 144 (41.4%) were infected with 1,018 total eyeworms. Eyeworm abundance (mean±SE) was 2.9±0.4 (range 0-64), with an intensity (mean±SE) of 7.1±0.6. Eyeworm prevalence was significantly higher in adult Northern Bobwhites (58.7%) than in juveniles (35.4%). Recent research suggests that eyeworms have the potential to cause cellular tissue damage to the eye, but it is unknown how these worms affect host survivability. This study further expands the regional distribution of O. petrowi in Northern Bobwhites in the Rolling Plains ecoregion and assesses the prevalence and abundance of infection across host age, host sex, and year. Further research is warranted on the life history of O. petrowi and assessing the impacts of eyeworms on their definitive host at individual and population levels.

The flight behavior and cover use of northern bobwhite (Colinus virginianus) have been examined in several studies, but the current data lack quantitative measures of how bobwhites respond to natural threats. We examined aspects of bobwhite behavior in response to 4 threat categories: researcher, hunter, raptor, and mammal. We found that bobwhite flight distance is best predicted by threat type and covey size, and bobwhite flight speed is best predicted by threat type. Bobwhites flushed by the hunter threat and the raptor threat selected for significantly taller obstruction at landing sites than was randomly available, with average heights of 6.2 cm taller (P = 0.034), and 38.1 cm taller (P < 0.001), respectively. Raptor‐flushed bobwhites also selected for significantly denser shrub cover (42.2%, P < 0.001) and a lower angle of obstruction (70.4°, P < 0.001) at landing points than was randomly available. In the process of data collection, we also observed bobwhite roost locations have lower visual height obstruction (7.3 cm, P = 0.03), lower shrub intercept (10.1%, P = 0.02), and greater angles of obstruction (18.5°, P = 0.005) than bobwhite diurnal locations; this may facilitate escape from nocturnal mammalian predators. Our results suggest that bobwhite escape strategies and cover use vary among threat types. These results support current management recommendations of creating a patchwork of vegetation covers for bobwhites but also suggest how a more complete understanding of bobwhite behavior would improve management and conservation efforts. © 2014 The Wildlife Society.