No abstract

Inheritance of gliadin components unique to three different varieties of common wheat (Triticum aestivum L.) was studied in F1 and F2 seeds of intervarietal crosses using protein patterns obtained by polyacrylamide gel electrophoresis in aluminum lactate buffer (pH 3.2). The patterns of F1 seeds of the crosses Cheyenne X Justin and INIA 66R X Justin evidenced all the bands present in the patterns of the parents; band intensities reflected gene dosage levels dependent on whether the contributing parent was maternal or paternal in accordance with the triploid nature of endosperm tissue. Most of the gliadin components examined segregated in accordance with control by a single dominant gene, but in two instances single bands in the one-dimensional electrophoretic patterns segregated in the F2 as expected if controlled by two genes. A method of two-dimensional electrophoresis was developed that resolved these apparently single bands into two components each, which could segregate independently. Linkage analysis provided evidence of codominant alleles and closely linked genes coding for gliadin protein components in both coupling and repulsion situations. The gliadin protein components seem to be coded for by clusters of genes located on chromosomes of homoeologous groups 1 and 6 in hexaploid wheats.

No abstract

No abstract

In extracts of developing seeds of wheat (Triticum aestivum L., cultivars Scout 66, Cheyenne, Nugaines and INIA 66R) examined by polyacrylamide gel electrophoresis, gliadin proteins appeared at 12-15 days post-anthesis with mobilities covering nearly the whole range found with mature seeds. Some differences among extractants [ 2~ dimethylformamide (DMF); 2111 urea; 60 % ethanol ; the aluminium lactate-lactic acid electrophoresis buffer, pH 3.21 in both intensity and occurrence of specific bands were observed. With immature seeds, 60% ethanol extracts produced a maximum number of bands and least background colour, but DMF and urea extracts showed bands from 12-to 15-day seeds at least as well as 60% ethanol extracts despite more background colour. Aluminium lactate buffer extracts occasionally yielded bands not found with other extractants, but gave poor extraction from immature seeds and relatively heavy background staining with mature seeds, especially in the w-gliadin (lowest mobility) region. A quantitative sodium dodecyl sulphate-electrophoresis procedurel was used to follow the formation of proteins in various molecular weight ranges (cv. Scout 66 only). At 12-18 days post-anthesis, synthesis of protein of molecular weight 30 000-37 000 accelerated, consistent with the onset and acceleration of formation of a-, /3-, and some y-gliadins. Protein in a band of 44 000 molecular weight formed in similar fashion. In other molecular weight ranges, gliadin bands could not be positively identified because of the complexity of the banding pattern of the total protein.

No abstract

A derivative of wheat gluten was prepared by treatment with phosphoric acid and urea at 140°C , an adaptation of a procedure used for phosphorylation of cellulose. The phosphorylated product was capable of absorbing about 200 times its weight of water to form a firm gel. This preparative method has several advantages over those previously described for making the gel-forming derivative by sulfation or phosphorylation.

No abstract