The Molecular Weight of Lysozyme after Reduction and Alkylation of the Disulfide Bonds

Interaction of aldehydes with collagen: effect on thermal, enzymatic and conformational stability

“…Formaldehyde is known to interact with the amino groups of protein, which is followed by further condensation with amides, and guanidyl groups [33,34]. An earlier study on the treatment of collagen with glyoxal had resulted in a decrease in lysine and hydroxylysine recoverable on hydrolysis, suggesting that the bond formed is relatively stable [29].…”

Human plasma-derived immunosuppressive factors having anti-lymphoma activity

“…After the 2-ME treatment, ling iodoacetamide per 0.38 ml 2-ME was added to the solution [13]; the pH was then adjusted to 8.5. After 1 h at 25 ° C the preparation was collected and washed with saline on a UM05 filter.…”

Inactivation of Lysozyme by Native Ovalbumin

“…Because lysozyme is inactivated by reducing agents (Fraenkel-Conrat et al, 1951) and because ovalbumin is the only egg white protein known to contain sulfhydryl groups (MacDonnell et al, 1951) tests were conducted to determine whether cysteine would cause inactivation under conditions similar to those used in the ovalbumin tests. Inactivation was found to occur readily at 60°C.…”