Interaction of aldehydes with collagen: effect on thermal, enzymatic and conformational stability

Porcine skin as a source of biodegradable matrices: alkaline treatment and glutaraldehyde crosslinking

“…The higher resistance to degradation of the nonsequentially crosslinked matrix can be associated to the impermeable effect that occurs by the GA polymerized in the The crosslinking can be attributed to the formation of Schiff base chemical bonds between GA and ε-amino groups of lysine and hydroxilysine present in the collagen matrix [16] . The results obtained from DSC curves also indicated that after alkaline hydrolysis the Td value decreased approximately 25 °C.…”

“…This increase occurs due to the alkaline hydrolysis of carboxyamides groups of Asn and Gln residues that increase the negative charges (carboxyl groups) [7] , resulting in an increase of solvation effect in the collagen and then, a higher quantity of absorbed water. After the GA crosslinking reaction, this water quantity increased further, due to the increase of negative charge density through the GA reaction with Lys and Hyl ε-amino groups of collagen matrix to form chemical bonds type Schiff bases (-CH=N) [16] . Comparing the differences in water loss between the 85GA45 and 85GA45S matrices, it can be observed that the matrix crosslinked non-sequentially (85GA45) shows a smaller water quantity than the matrix sequentially crosslinked (85GA45S), suggesting that 85GA45 is more impermeable.…”

“…Chemical crosslinking can result in materials which are permanently crosslinked [13] , and the in vivo degradation rate of these materials may be controlled [14] . Glutaraldehyde (GA) is a crosslinking agent used in collagen matrices that do not affect biocompatibility and increase biological stability [15,16] . The objective of this study was the preparation and characterization of collagen type I matrices submitted to an alkaline hydrolysis and GA crosslinking.…”

Introductory Chapter: Multi-Aspect Bibliographic Analysis of the Synergy of Technical, Biological and Medical Sciences Concerning Materials and Technologies Used for Medical and Dental Implantable Devices

“…Different synthetic and natural polymers were used for this purpose, including polyethylene glycol (PEG), and copolymers containing PEG [486,510], hyaluronic acid (HA) [511] after an oxidation reaction through HA-tyramine conjugates [505] and as a result of the formation between HA-SH [492,512] and Michael addition [491,513], collagen and gelatin hydrogels mostly cross-linked using glutaraldehyde, genipin or water-soluble carbodiimides [513][514][515], chitosan [516][517][518][519], dextran 192 [520,521] and alginate [522]. Hydrogels were used for reconstruction of the retina [523], ligament [524], fatty tissue [465], kidneys [525], muscles [526], blood vessels [527,528], and also heart, neural cells, invertebral discs, bones and gristle [459].…”

Immobilization of collagen peptide on dialdehyde bacterial cellulose nanofibers via covalent bonds for tissue engineering and regeneration

“…It is well known that aldehydic groups can covalently cross-link with collagen by amino functional groups of the protein. 31,32 Furthermore, pH can affect the zeta potential and isoelectric point (IEP, the pH when zeta potential =0 mV) of materials. 33 The zeta potential and isoelectric point with respect to the pH value of DBC and Col-p are shown in Figure 7.…”