No abstract

No abstract

In this work, the effectiveness of different enzymatic techniques for cell wall disruption of Haematococcus pluvialis for the extraction of carotenoids and subsequent encapsulation of extracts in the co-polymer poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) using the Solution Enhanced Dispersion by Supercritical fluids (SEDS) technique was investigated. Glucanex(®) performed best compared with Lyticase(®) and Driselase(®). The conditions for enzymatic lysis using this enzyme preparation were established as a pH of 4.5, a temperature of 55 °C, an initial activity of β-1,3-glucanase of 0.6 U mL(-1) and a reaction time of 30 min. Enzymatic lysis assisted by ultrasound without biomass freezing was shown to be a promising and simple one-step technique for cell wall disruption, reaching 83.90% extractability. In the co-precipitation experiments, the highest encapsulation efficiency (51.21%) was obtained when using a higher biomass to dichloromethane ratio (10 mg mL(-1)) at the carotenoid extraction step and a lower pressure of precipitation (80 bar). In these conditions, spherical particles in the micrometer range (0.228 μm) were obtained.

No abstract

No abstract

No abstract

No abstract

ABSTRACT. Several micronization processes based on supercritical fluids have been developed. These processes can be classified according to the role of the supercritical fluid in the process: solvent, solute or co-solvent, and anti-solvent. Application of supercritical fluids as alternative to traditional micronization methods and the growing demand of the industrial sector for new technologies motivated this review. In this context, the objective of this work was to present the operating principles of the micronization process by means of supercritical fluids and the effects of the main process variables on particles characteristics. The review continues with an update of current experimental data presented in the literature in the period from 2008 to 2012.Keywords: supercritical fluids, micronization process, review.Processos de micronização utilizando tecnologia supercrítica: uma breve revisão sobre projeto de processos RESUMO. Vários processos de micronização baseados na tecnologia supercrítica têm sido desenvolvidos. Tais processos podem ser classificados conforme o papel do fluido supercrítico no processo: solvente, soluto ou co-solvente e anti-solvente. O presente trabalho de revisão foi motivado pela cresecnte demanda do setor industrial por técnicas alternativas e também devido a aplicabilidade dos fluidos supercríticos. Neste contexto, o principal objetivo deste trabalho é apresentar os princípios operacionais referentes a micronização com fluidos supercríticos e os efeitos das principais variáveis de processo nas características das partículas produzidas. O presente trabalho de revisão cobre os avanços experimentais reportados na literatura no período de 2008-2011.Palavras-chave: fluído supercrítico, processo de micronização, revisão.