The action of high-intensity ultraviolet pulse laser radiation on a 161 bp fragment of pBR 322 DNA (EcoRIlwspI fragment) was studied. At doses up to 5 x lOI photons/cm2 the N-gly~sidic bond splitting is negligible. The action of hot piperidine on irradiated DNA leads to chain splitting at the residues, modified via biphotonic processes. The modification and, hence, splitting efliciences depend on the type of base (G > T > A > C) and on its position in the sequence. Preferentially modified bases in the opposite strands of double-stranded DNA belong, mainly, to the same or adjacent base pairs. Residues in the Pribnow box are modified considerably less, than in Ihe sequences, immediately upstream and downstream. This approach seems to be useful in footp~nting of DNA secondary structure peculiarities and alterations, conjugated with the functional role and state of the respective fragment.Foo tprinting DNA secondary structure DNA splitting Laser ultraviolet irradiation PlasmidpBR 322
The target model of the NRNU MEPhI provides, in particular, the transition to the use of distance learning platforms, integrated with the international educational environment and social learning networking. A special place in education network is allocated to computer tools to support the learning process. These tools include Cyber Learning Platform for Nuclear Education and Training (CLP4NET) related to the automated monitoring systems and support of the learning process. NRNU MEPhI pays particular attention to Control and managed content (CMC), virtual labs and PC-based simulators of nuclear power plant (NPP), and especially when they are used as part of CLP4NET.
Keywords-Cyber Learning Platform for Nuclear Education;Control and managed content; virtual laboratories; PC-based simulators of nuclear power plant.
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