A simple, sensitive and reproducible spectrophotometric method was developed for the determination of aripiprazole in pure form and in pharmaceutical formulation. It has an absorption maximum at 219 nm and obeys beer's law in the concentration range 2- 10 µg mL-1. Results of analysis were validated statistically and by recovery studies. The apparent molar absorptivity and sandell's sensitivity were 5.2 x 105L mol-1cm-1and 8.4 x 10-3µg cm-2, respectively. The slope and intercept of the equation of the regression line are 0.0035 and 0.1155 respectively. Correlation coefficient was found to be 0.9998. This method is successfully employed for the determination of aripiprazole in pharmaceutical preparation.

Two experiments were conducted each using one batch of six Landrace littermate, entire male, pedigree pigs in a Latin-square change-over study of the effects of energy intake on nitrogen and energy metabolism over the range 33 to 88 kg live weight. One animal from each litter was slaughtered at 33 kg body weight to obtain initial body composition data. Five feeding levels (80, 100, 120, 140 and 160g/kg M0·63) were used during five consecutive metabolism trials each of 11-days duration, excreta being collected during the last 7 days. The pigs were housed in individual metabolism cages and the diets were offered in liquid form (approx. 300 g dry matter (DM) per kg) twice daily at 09.00 and 16.00 h. Heat production was measured for 1 day during each balance period in an open-circuit respiration chamber. The average daily gain, nitrogen retention, heat production and energy retention increased linearly (P < 0·001) with increasing metabolizable energy (ME) intake. The relationship between energy intake and protein deposition was linear up to levels above the normal ad libitum consumption of energy. Protein deposition potential of these high genetic potential pigs was at least 200 g/day, and tended to be constant between 35 and 85 kg live weight. From the combined results of experiments 1 and 2, the energy requirement for maintenance was 0·982 MJ ME per kg M0·63 per day and the decrease in protein deposition was approximately 6 g/MJ reduction in ME within the range of practical energy intakes.

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1990). Effect of protein intake on energy and nitrogen balance and chemical composition of gain in growing boars of high genetic potential. ABSTRACT The effects of dietary protein (151 to 282 g crude protein per kg dry matter (DM)) and lysine (8-5 to 16-4 g/kg DM) on the carcass composition and energy metabolism of entire male pigs, given food close to appetite, was studied from 33 to 88 kg. Four replicates (three Landrace and one Duroc) of four littermates were used. Energy and nitrogen (N) balances were conducted at approximately 35 to 43, 58 to 65 and 78 to 85 kg and body composition was determined at slaughter. There was no effect of dietary treatment on the crude protein content of the empty body (EBW) but the DM (P < 0-001), fat (P < 0-001) and ash (P < 0-05) proportions and fat : protein ratio in EBW (P < 0-01) increased with decreasing dietary protein level. The mean maximum protein retention was 183 g/day. Retention of protein (P < 0-05) and proportion of protein energy in the gain (P < 0-01) decreased linearly and fat retention (P < 0-001) and energy content of the gain (P < 001) increased with decreasing dietary protein. The N retention values calculated from balance data were proportionately 0-21 higher at high protein intakes (509 g/day) and 0-056 higher at low protein intakes (329 g/day) than the values obtained by slaughter. Using the ideal protein system the value for the efficiency of utilization of apparently digested ideal protein for protein deposition (a 2 ) fell linearly (P < 0-001), based on the slaughter data, as dietary protein content increased. Heat production, calculated from slaughter data, was proportionately 0-07 higher than that measured by indirect calorimetry and 0-17 higher than the computed value for heat production using the standard values of energy costs for maintenance and for protein and fat deposition.

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