The aim: To determine the dynamics of changes in metric parameters of the duct system of rat submandibular glands in normal conditions and chronic ethanol intoxication.
Materials and methods: 50 albino outbred rats were involved in the experiment. 10 animals were assigned in the control group, 40 animals – in the experimental group.
Animals were sacrificed on 5, 9, 12 and 30 days by overdose of thiopental anesthesia. Pieces of the submandibular glands were embedded into epon-812 according to the conventional technique.
Results: On day 5 of the experiment the lumen diameter of intercalated duct reduced by 9,15 % (р <0,05). The lumen diameter of the striated ducts was by 5,29 % significantly greater than the values in controls (р <0,05). The lumen diameter of the granular ducts reduced by 2,45 % (р <0,05). On day 30 of the experiment the height of the epithelial cells of the intercalated ducts was by 8,47 % significantly less (р <0,05), the height of the epithelial cells of the striated ducts was by 12,27 % less (р <0,05) and the height of the epithelial cells of the granular ducts was by 11,96 % less (р <0,05) than the values in controls.
Conclusions: No recovery of parameters occurs by day 30 of the experiment, indicating the depletion of the secretory epithelium of the duct system, due to dystrophic changes caused by vascular disorder in the microvasculature.
The aim of the study was to determine the stroma structural features in the major salivary glands of rats in normal state. The study was carried out on 20 adult white male Wistar rats with body weight 260-300 grams, aged 10-12 months. Histological examination of the parotid stroma determined that the intralobular connective tissue was represented by amorphous substance, collagen fibers and fibroblast processes between adjacent terminal parts. The fibroblast bodies were located in the nodular interstitial compartments − the contact points of 3-4 terminal parts of the gland lobules as well as collagen fibers and blood vessels of the hemomicrocirculatory bed − capillaries and postcapillaries. Postcapillaries and venules were revealed in periductal connective tissue. The local protective barrier of the major salivary glands stroma in rats is predominantly represented by plasmocytes and macrophages in the interacinar interstitium as well as macrophages and mast cells in the periductal connective tissue.
The morphometric study conducted has revealed that the effect of a 1% methacrylic acid-methyl ester solution affects the resistive section of the microvasculatory bed of the submandibular salivary gland lobes. The study was conducted on 30 white outbred male rats, 10 of which made up the control group of animals and 20 animals made up experimental group, whose oral mucosa was treated with 1% methacrylic acid-methyl ester solution for 30 days. After euthanasia of animals in 14 and 30 days, samples of the submandibular gland were embedded into epon-812. Semi-thin sections were stained with polychrome dye. A quantitative analysis of the results of the morphometric study and statistical processing of the morphometric data was performed using standard statistical methods using the Excel program. The outer diameter and lumen diameter of arterioles were measured. The thickness of the vascular wall was calculated using the formula VWTh = Dz-Dt / 2. On the 14th day, in response to the action of 1% methacrylic acid-methyl ester solution, the average outer arteriole diameter decreased by 13.04%, the diameter of the of arteriole lumen by 20.77%. There is a significant increase in the average thickness of the vascular wall by 5.45%, the value of which was 2.90 microns. On the 13th day of the study, it was found out that the average values of the outer diameter of the arterioles increased by 5.04% (p <0.05), compared with the control group. The lumen diameter was significantly larger by 15% that the values in the control group of animals, and the average vascular wall thickness was significantly lower by 18.91% than the values in the control group of animals (p <0.05). Normalization of indicators for the 30th day has not been defined. Thus, the effect of 1% methacrylic acid-methyl ester solution on the arterioles of the submandibular salivary gland lobes is characterized by vascular spasms in the early stages, and vascular dilatation in the later stages of the experiment.
Introduction: The state of the segments of the microvasculature, especially exchange one, has a significant effect on the organs’ functions. The results of it’s research can be
objectified by morphometric method.
The aim: to determine the dynamics of changes in metric indices of the exchange segment of microvasculature of rats’ submandibular glands in normal conditions and in
chronic ethanol intoxication.
Materials and methods: 45 albino rats were involved into study. Intact group (n=5) animals were administered with NaCl isotonic solution 4 times a day directly into the
stomach. Experimental group (n=40) animals were administered with 40° ethanol 4 times a day directly into the stomach. Animals were sacrificed on 5, 9, 12 and 30 days by
overdose of thiopental anesthesia. Lobules of submandibular glands were embedded into epon-812 according to standard procedure.
Results: On day 5 of the experiment the outer diameter of the capillary wall significantly reduced (4,91±0,02 μm), that is 19,5 % less than the values in controls (р<0,05).
On day 9 of the experiment it was 15,2 % lower than the value of control group (р>0,05). On day 30 of the experiment the outer capillary diameter was 8 % lower compared
to controls (р<0,05).
Conclusions: The early observation showed vasodilatation, confirmed by the constriction of the outer diameter and lumen diameter, accompanied by the thickening of the
vascular wall under the influence of chronic ethanol intoxication. The indices did not come to normal values by the 30-th day of the experiment.
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